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Bpsbioscience/PD1[Biotinylated]:PDL2 Inhibitor Screening Assay Kit/72006/96 reactions188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Bpsbioscience/PD1[Biotinylated]:PDL2 Inhibitor Screening Assay Kit/72006/96 reactions
Background:
CellsignalingthroughthePD-1receptoruponbindingthePD-L2ligandattenuatesimmuneresponsesandisexploitedbybothtumorsandviruses.
Description:
ThePD-L2InhibitorScreeningAssayKitisdesignedforscreeningandprofilinginhibitorsofthissignaling.Thekeytothiskitisthehighsensitivityofdetectionofbiotin-labeledPD-1bystreptavidin-HRP.Onlyafewsimplestepsonamicrotiterplatearerequiredfortheassay.First,PD-L2iscoatedona96-wellplate.Next,PD-1isincubatedwithPD-L2ontheplate.Finally,theplateistreatedwithstreptavidin-HRPfollowedbyadditionofanHRPsubstratetoproducechemiluminescence,whichcanthenbemeasuredusingachemiluminescencereader.
SuppliedAs:
Thiskitcomesinaconvenient96-wellformat,withbiotin-labeledPD-1,purifiedPD-L2,streptavidin-labeledHRP,andassaybufferfor100bindingreactions.
Contraindications:
DMSO>1%,strongacidsorbases,ionicdetergents,highsalt.
Format:

Storage/Stability:
Stableatleast6monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Reference(s):
1.Lazar-Molnar,E.,etal.ProcNatlAcadSciU.S.A.2008,105:10483-10488.
2.Keir,M.E.,etal.Annu.Rev.Immunol.2008,26:677-704.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
CellSurfaceReceptor/Single-passTransmembraneProtein
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.

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