Bpsbioscience/Human Interleukin18/90183B/25 µg188bio精品生物—专注于实验室精品爆款的电商平台

Species:Human

HostSpecies:E.coli

MW:18kDa

Genbank#:Q14116

a.a:37–193

Background:

IL-18issynthesizedasabiologicallyinactiveprecursorproteinthathaslimitedbiologicalactivity.TheproformofIL18isprocessedbyoneofthecaspases,Caspase-1,togeneratethe18kDabioactivemolecule.IL-18isproducedduringtheacuteimmuneresponsebymacrophagesandimmaturedendriticcells.Interleukin-18isexpressedbyavarietyofimmuneandnon-immunecells,includingmonocytesandmacrophages.IL-18isoneofthepro-inflammatorycytokines.TheactivitiesofIL-18appeartobespecies-specific.AnimportantfunctionofIL-18istheregulationoffunctionallydistinctsubsetsofT-helpercellsrequiredforcellmediatedimmuneresponses.IL-18functionsasagrowthanddifferentiationfactorforTh1cells.IL-18up-regulatesFASligandmediatedcytotoxicactivitiyofmurinenaturalkillercells.IL-18ispartofacomplexregulatorycircuitinvolvedincausingcelldeathbyapoptosis.

Description:

RecombinantInterleukin-18isadisulfide-linkedmonomerproteinconsistingof158aminoacidresidues,migratesasanapproximately18kDaproteinundernon-reducingandreducingconditionsinSDS-PAGE.OptimizedDNAsequenceencodinghumanInterleukin-18maturechainwasexpressedinE.coli.

UniProt

Q14116

Synonym(s):

Interleukin18,Interferon-Gamma-InducingFactor,IGIF,IL-18,IL18,IFN

Purity:

≥98%bySDS-PAGEandHPLC

EndotoxinLevel:

<0.1ng/µg(1EU/µg),usingtheLALgelclotmethod.

BiologicalActivity:

TheED₅₀wasdeterminedbythedose-dependentproliferationofhumanPBMCco-stimulatedwithIL-12cellswasfoundtobeintherangeof5ng/ml.

Formulation:

Lyophilizedfrom0.2µmfilteredPBS,pH7.

Format:

lyophilizedprotein

Reconstitution:

Reconstituteat0.1-1.0mg/mlindistilledwater.Thissolutioncanthenbedilutedintootherbuffers.Tomaximizeproductcollectionfromvialsurface,vortexbrieflyandthenspindowntorecollecttheliquid.

Storage/Stability:

Thelyophilizedproteinisstableforatleast2yearsfromdateofreceiptwhenstoredat-20°C.Uponreconstitution,storeinworkingaliquotsat+4°Cforuptoonemonth,orat-20°Cforuptosixmonths,inthepresenceofacarrierprotein.Avoidrepeatedfreeze/thawcycles.

Reference(s):

1.TsutsuiH,NakanishiK.Immunotherapy.2012Dec,4(12):1883-94.
2.BarkerBR,etal.CurrOpinImmunol.2011Oct,23(5):591-7

Warning(s):

Avoidfreeze/thawcycles

AminoAcidSequence:

YFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED

ScientificCategory:

Cytokine/GrowthFactors

ProductType:

RecombinantProtein

Datashownislot-specific.Contactusforspecificinformationonotherlots.

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Bpsbioscience/Human Interleukin18/90183B/25 µg