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Bpsbioscience/DNMT3B/DNMT3L Complex, Histag, GSTtag/51104/10 µg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Bpsbioscience/DNMT3B/DNMT3L Complex, Histag, GSTtag/51104/10 µg
Species:Human
HostSpecies:Sf9insectcells
MW:86kDa(complex)
Genbank#:NM_006892(DNMT3B),NM_013369(DNMT3L)
Tag(s):N-terminalHis,N-terminalGST-tag
a.a:564–853(end),DNMT3b;160–387(end),DNMT3L
Description:
ComplexofhumanDNAmethyl-transferase3B(DNMT3B),GenBankAccessionNo.NM_006892,a.a.564-853(end)withN-terminalHis-tag,MW=34kDa,andDNMT3L(GenBankAccessionNo.NM_013369),a.a.160-387(end),withanN-terminalGST-tag,MW=52kDa,co-expressedinabaculovirus-infectedSf9cellexpressionsystem.
UniProt
Q9UBC3(DNMT3B),Q9UJW3(DNMT3L)
SpecificActivity:
≥0.52pmol/min/µg.
AssayConditions:
AssayedusingBPSBioscienceDNMT3BAssayKit,Cat.#52034.Add50µlreactionmixcontainingDNMT3B/DNMT3LtowellscoatedwithDNMTsubstrate.Incubate2hrat37°C.Addantibodyagainst5-methylcytosine,thenHRP-labeledsecondaryantibodyfollowedbychemiluminescentdetection.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,16mMglutathione,and20%glycerol
Format:
Aqueousbuffersolution
Storage/Stability:

>6monthsat–80°C.

Application(s):
UsefulforthestudyofDNAmethylation,screeninginhibitors,andselectivityprofiling.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Methyltransferase
ProductType:
RecombinantProtein
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