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Bpsbioscience/TNKS2 Histone Ribosylation Colorometric Assay Kit/80583/96 reactions188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Bpsbioscience/TNKS2 Histone Ribosylation Colorometric Assay Kit/80583/96 reactions
Description:
TheTNKS2HistoneRibosylationColorimetricAssayKitisdesignedtomeasureTankyrase2(TNKS2)activityforscreeningandprofilingapplications.TNKS2catalyzestheNAD-dependentadditionofpoly(ADP-ribose)tothesubstrateproteins.ThekeytotheTNKS2HistoneRibosylationAssayisthebiotinylatedNAD+substrate.Withthiskit,onlythreesimplestepsarerequiredforTNKS2reactions.First,histoneproteinsarecoatedona96-wellplate.Next,thebiotinylatedNAD+substrateisincubatedwithanassaybufferthatcontainstheTNKS2enzyme.Finally,theplateistreatedwithstreptavidin-HRPfollowedbyadditionofthecolorimetricHRPsubstratetoproducecolorthatcanthenbemeasuredusingaUV/Visspectrophotometermicroplatereader.
Synonym(s):
TNKS2,poly(ADP-ribose)polymerase5b,poly(ADP-ribosyl)transferase5b,PARP5B,PARP5C,TRF1-interactingankyrin-relatedADP-ribosepolymerase2,TANK2,tanykyrase-2,TNKL
SuppliedAs:
TheTNKS2assaykitcomesinaconvenient96-wellformat,withpurifiedTNKS2enzyme,histonemixture,andPARPassaybufferfor100enzymereactions.
Format:

COMPONENTS:

Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol
Storage/Stability:
Stableatleast6monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.
Reference(s):

Brown,J.A.,Marala,R.B.J.Pharmacol.Toxicol.Methods200247:137-41.

ApplicationReference(s):
1. SmallMoleculesInspiredbytheNaturalProductWithanolidesasPotentInhibitorsofWntSignaling (2017)

Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
PARP
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.

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