Deprecated: Required parameter $cat_id follows optional parameter $type in /www/wwwroot/ebimall.com/systems/hong.php on line 2088

Deprecated: Required parameter $where follows optional parameter $tree_id in /www/wwwroot/ebimall.com/systems/hlb.php on line 3505
Bpsbioscience/Anti–ER alpha monoclonal antibody/25221/100 µl188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
您好,欢迎您进入188进口试剂采购网网站! 服务热线:4000-520-616
蚂蚁淘商城 | 现货促销 | 科研狗 | 生物在线

Bpsbioscience/Anti–ER alpha monoclonal antibody/25221/100 µl
HostSpecies:Mouse
Clonality:monoclonal
Background:
Theestrogenreceptoralpha(ERalpha,UniProt/Swiss–ProtentryP03372)belongstothefamilyofnuclearhormonereceptors,whichareligand–activatedtranscriptionfactors.Theyareimportantfortheregulationofgeneexpression,cellularproliferationanddifferentiation,sexualdevelopmentandreproductivefunction.Estrogenreceptorsarealsoinvolvedinpathologicalprocessessuchasbreastcancer,andosteoporosis.ERalphacanregulatetranscriptionbydirectbindingtoestrogenresponseelements(EREs)intheDNAorbyinteractionwithothertranscriptionfactors.ItmayalsoformaheterodimerwithERbeta.
Description:
MonoclonalantibodyraisedinmouseagainsthumanERalpha(estrogenreceptoralpha),usingasyntheticpeptide
Synonym(s):
ESR,ESR1,ESRA,NR3A14
AssayConditions:

ChIP-seqresultsobtainedwiththemonoclonalantibodydirectedagainstERalpha
ChIPwasperformedwiththemonoclonalantibodyagainstERalpha(Cat.#25221)onshearedchromatinfromMCF7cellstreatedfor1hourwithestradiol.TheimmunoprecipitatedDNAwasanalyzedwithanIlluminaGenomeAnalyzer.Librarypreparation,clustergenerationandsequencingwereperformedaccordingtothemanufacturersinstructions.The36bptagswerealignedtothehumangenomeusingtheELANDalgorithm.Figure1showstheobtainedpeaksneartheTFF1geneonchromosome21(figure1A),theGREB1andHAAOgenesonchromosome2(figure1BandC),andtheZNF185geneontheX-chromosome(figure1D).

Concentration:
100µl
Formulation:
Ascitesfluidfrommousecontaining0.05%azide
SpeciesReactivity:
Human
CrossReactivity:
Doesnotreactwithchicken;otherspeciesnottested
Purification:
Ascitesfluid
Immunogen:
syntheticpeptide
Format:
aqueoussolution
Storage/Stability:

Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.

Application(s):
ChIP/ChIP–seq(2.5µl)
ELISA(1:500–1:5000)
WB(1:500–1:5000)
GelSupershift(1:10–1:20)
Immunochemistry(1:500–1:5000)
IP(1:200–1:5000)
Notes:
TheoptimalantibodyamountperIPshouldbedeterminedbytheend–user.Werecommendtesting1–5µgperIP
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Antibodies
ProductType:
Antibody
Datashownislot-specific.Contactusforspecificinformationonotherlots.

新闻动态
行业前沿
技术文章
最新产品

188进口试剂采购网 www.188bio.cn -中国试剂网,试剂网,化学试剂网,国药试剂,抗体公司,试剂公司,试剂盒公司,苏州试剂公司,北京化学试剂公司,天津化学试剂,试剂商城,试剂代理,流式抗体 细胞库查询 sitemap