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Bpsbioscience/HSP90β (Nterminal) Assay Kit/50299/384 reactions188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Bpsbioscience/HSP90β (Nterminal) Assay Kit/50299/384 reactions
Background:

Hsp90βisamolecularchaperonewithessentialfunctionsinmaintainingtransformation.InhibitionofHsp90βfunctionshasbeenshowntoplayaroleintumorigenesisanddiseaseprogression.

Description:
TheHSP90β(N-terminal)AssayKitisdesignedforidentificationofHSP90βinhibitorsusingfluorescencepolarization.TheassayisbasedonthecompetitionoffluorescentlylabeledgeldanamycinforbindingtopurifiedrecombinantHSP90β.ThekeytotheHSP90βAssayKitisthefluorescentlylabeledgeldanamycin.Usingthiskit,onlyonesimplesteponamicrotiterplateisrequiredforHSP90βreactions.ThefluorescentlylabeledgeldanamycinisincubatedwithasamplecontainingHSP90βenzymetoproduceachangeinfluorescentpolarizationthatcanthenbemeasuredusingafluorescencereader.
UniProt
P08238
Synonym(s):
HSP90b,HeatShockProtein90b,HSP90beta,HSP90β
SuppliedAs:
TheHSP90βinhibitorscreeningassaykitcomesinaconvenient384-wellformat,withpurifiedHSP90βenzyme,fluorescentlylabeledgeldanamycinandHSP90assaybufferfor384enzymereactions.
Contraindications:
DMSO>1%,strongacidsorbases,ionicdetergents,highsalt
Format:

COMPONENTS:

Instructionsforuse:
Seedatasheetfordetailedprotocol.
Storage/Stability:

Stableforatleast12monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.

Application(s):
GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.
Reference(s):
1.Kim,J.,etal.JBiomolScreening2004,9(5):375-381.
2.Howes,R.,etal.AnalBiochem2006,350:202-213.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
HeatShockProteins
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.

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