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Bpsbioscience/HIPK4, GSTtag/40111/10 µg
Species:Human
HostSpecies:Sf9insectcells
MW:110kDa
Genbank#:NM_144685
Tag(s):N-terminalGST-tag
a.a:fulllength
Background:
HIPK4orhomeodomaininteractingproteinkinase4isaserine/threonineproteinkinasethatcanactasaco-repressoroftranscriptionfactors(1).HIPK4hasbeenshowntophosphorylatethehumanp53proteinatserine9,bothinvitroandinvivowhichisimportantforp53mediatedtranscriptionalrepression.RT-PCRanalysisshowedmoderateexpressionofHIPK4inlungandwhiteadiposetissueandweakexpressioninbrainandliver.HIPK4expressionhasalsobeenobservedinvariouscancercelllines(2).
Description:
HumanHIPK4(homeodomain-interactingproteinkinase4),GenBankAccessionNo.NM_144685,fulllength,withN-terminalGSTtag,MW=110kDa,expressedinSf9insectcellsviaabaculovirusexpressionsystem.
Synonym(s):
FLJ32818
Purity:
≥70%
SpecificActivity:
202pmol/min/µg
AssayConditions:
5mMMOPS,2.5mMβ-glycerophosphate,5mMMgCl2,1mMEGTA,0.4mMEDTA,0.05mMDTT,2mMATP.IncubateHIPK4with0.25mg/mlMyelinbasicProtein(substrate)and50?M[33P]-ATPat30°Cfor15minutes,thenspotreactiononphosphocellulosepaper,fixin1%phosphoricacid,andassaywithascintillationcounter.
Formulation:
50mMTris-HCl,pH7.5,150mMNaCl,0.25mMDTT,0.1mMEGTA,0.1mMEDTA,0.1mMPMSF,25%glycerol.
Format:
Aqueousbuffersolution
Storage/Stability:

>6monthsat-80°C.

Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Arai,S.etal:Novelhomeodomain-interactingproteinkinasefamilymember,HIPK4,phosphorylateshumanp53atserine9.FEBSLett.2007;11;581(29):5649-57.
2.DiStefano,V.etal:Homeodomain-interactingproteinkinase-2activityandp53phosphorylationarecriticaleventsforcisplatin-mediatedapoptosis.Exp.Cell.Res.2004;15;293(2):311-20.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Serine-Threonine
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.

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