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Bpsbioscience/B71 (CD80), Fc fusion, Biotinlabeled (Human) HiP™/71114/50 µg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Bpsbioscience/B71 (CD80), Fc fusion, Biotinlabeled (Human) HiP™/71114/50 µg

Species:Human
HostSpecies:HEK293
MW:52.5kDa
Genbank#:NM_005191
Tag(s):C-terminalFcfusion(HumanIgG1),C-terminalAvi-tag™
a.a:35-242
Description:

HumansecretedB7-1-Fcfusionprotein,alsoknownasCD80,withC-terminalAvi-tag™.GenBankAccessionNo.NM_005191,a.a35-242expressedinaHEK293cellexpressionsystemandenzymaticallybiotin-labeledusingAvi-tag™technology.MW=52.5kDa(monomer).ThisproteinrunsatahigherM.W.bySDS-PAGEduetoglycosylation.

UniProt
P33681
Synonym(s):
CTLA-4Counter-ReceptorB7.1
AssayConditions:
ReactiondoneaccordingtoCTLA4:B7-1[biotin]assaykit(Catalog#72009)protocol.BufferisPBS+0.1%BSA.BlockingbufferisSuperblock+0.05%Tween-20.CoatproteinwasaddedONat4deg100ng/well.Variousconcentrationsofbiotinylatedligand(0-8ng/ul,0.5ng/ulistherecommendedkitcondition)addedfor2hatRT.Strep-HRPaddedfor1hatRT.
Formulation:
40mMTris,pH8.0,110mMNaCl,2.2mMKCl,and20%glycerol.
Format:
Aqueousbuffersolution
Storage/Stability:
Atleast6monthsat–80°C.
Application(s):
Usefulforthestudyofprotein-proteininteractionandinvitroandinvivoproteinfunctionstudies.
Reference(s):

1.Byrum,J.N.,VanKomen,J.S.,Rodgers,W.,J.Immunol.2013Sep15;191(6):3073-3081.

2.Legat,A.,etal.,FrontImmunol.2013Dec19;4:455.

Notes:

HiP™indicatesahighpurityproteinwith≥90%purityasmeasuredbygelfiltration.

Warning(s):
Avoidfreeze/thawcycles.Proteinmaybedilutedto≥100μg/mlinPBS+glycerolandstoredat-80°C.
ScientificCategory:
Immunotherapy
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.

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