Bpsbioscience/Human Interleukin7/90198A/2 µg188bio精品生物—专注于实验室精品爆款的电商平台

Species:Human

HostSpecies:E.coli

MW:17kDa

Genbank#:P13232

a.a:26–177

Background:

IL-7issecretedconstitutivelyintotheconditionedmediumofadherentbonemarrowstromalcellsandthymiccells.MouseandhumankeratinocyteshavebeenshownalsotoexpressandsecreteIL-7.Human(152aminoacids)andmurineIL-7(129aminoacids)show60%sequencehomologyattheproteinlevel.ThehumanIL-7receptor,designatedCD127,isanintegralstronglyglycosylatedmembraneproteinof76kDaexpressedonactivatedT-cells.IL-7receptorsareexpressedonpre-B-cellsandtheirprogenitorsandbonemarrowmacrophages,buttheyarenotexpressedonmatureB-cells.FunctionalIL-7receptorsarealsofoundonthecellsurfaceofmultiphenotypic,biphenotypic,andimmaturelymphoidprogenitorsofB-cellswiththegenearrangementoftheheavyimmunoglobulinchainsuchasthoseobservedinthegermline.

Description:

RecombinanthumanIL-7isadisulfide-linkedmonomericproteinconsistingof153aminoacidresidues,andmigratesasanapproximately17kDaproteinundernon-reducingconditionsandreducingconditionsinSDS-PAGE.OptimizedDNAsequenceencodingHumanInterleukin-7maturechainwasexpressedinE.coli.

UniProt

P13232

Synonym(s):

IL-7,Interleukin7,IL7

Purity:

≥97%bySDS-PAGEandHPLC

EndotoxinLevel:

<0.1ng/µg(1EU/µg),usingtheLALgelclotmethod.

BiologicalActivity:

TheED₅₀determined bydose-dependentstimulationoftheproliferationofmurine2E8cellsis≤0.5ng/ml,correspondingtoaspecificactivityof≥2x10⁶units/mg.

Formulation:

Lyophilizedfroma0.2µmfilteredPBSsolution,pH4.5

Format:

lyophilizedprotein

Reconstitution:

Reconstituteat0.1-1.0mg/mlindistilledwater.Thissolutioncanthenbedilutedintootherbuffers.Tomaximizeproductcollectionfromvialsurface,vortexbrieflyandthenspindowntorecollecttheliquid.

Storage/Stability:

Thelyophilizedproteinisstableforatleast2yearsfromdateofreceiptwhenstoredat-20°C.Uponreconstitution,storeinworkingaliquotsat+4°Cforuptoonemonth,orat-20°Cforuptosixmonths,inthepresenceofacarrierprotein.Avoidrepeatedfreeze/thawcycles.

Reference(s):

1.J.Exp.Med.,Sep2009,206:2111-2119.
2.J.Immunol.,Sep2009,183:3130-3138.
3.Blood,Aug2009,114:1768-1775.

Warning(s):

Avoidfreeze/thawcycles

AminoAcidSequence:

DCDIEGKDGKQYESVLMVSIDQLLDSMKEIGSNCLNNEFNFFKRHICDANKEGMFLFRAARKLRQFLKMNSTGDFDLHLLKVSEGTTILLNCTGQVKGRKPAALGEAQPTKSLEENKSLKEQKKLNDLCFLKRLLQEIKTCWNKILMGTKEH

ScientificCategory:

Cytokine/GrowthFactors

ProductType:

RecombinantProtein

Datashownislot-specific.Contactusforspecificinformationonotherlots.

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Bpsbioscience/Human Interleukin7/90198A/2 µg