absoluteantibody/Anti-i-motif DNA [iMab]/100 μg/Ab01462-23.0

AlternativeName(s)ofTarget:i-motif;intercalatedmotif;intercalatedDNAmotif;imotif;DNAi-motif;humantelomerei-motif;hTeloi-motif;imotif;imotiv;imotive;i-motiv;i-motiveImmunogen:ThisantibodyfragmentwasselectedviaphagedisplayfromtheGarvan-2humansingle-chainvariablefragment(scFv)library.

Specificity:ThisantibodyfragmentisspecifictotheDNAi-motif(Kd=59nM),whichisafour-strandedDNAstructurepotentiallyinvolvedinDNAregulationandformedinthecytosine-richregions.iMabantibodystructurewasshowntobeabletorecognisevariousi-motifs(1G22,1A83,c-MYC;RET,VEGF)locatedindifferentregionsofDNA(telomeres,centromeres,promotersofproto-oncogenes)whichmighthavedistincnucleotidesequences.Atthesametime,iMabantibodyfragmentdoesnotbindtomanyotherstructureswhicharesimilartoi-motifs,suchasdifferentDNAG4quadruplexforms,dsDNA;hairpinDNA;miRNAormutanthumantelomereswithoutthei-motif(ZerAATietal.,2018).ThissuggeststhatiMabisani-motifstructure-specificantibody.

ApplicationNotes:iMabisahighlysensitiveandspecificantibodyfragmentdirectedagainstDNAi-motifsanditisrecommendedfortheidentificationoftheseDNAstructures.Itsspecificitytowardsvarioustypesofthei-motifwasconfirmedviaELISAandbio-layerinterferometry(BLI)whichshowedthatiMabdidnotbindtoanysimilarstructuresandmolecules(mostimportantly,itdidnotbindtovariousDNAG4quadruplexforms,suchaspromoterG4sc-MYC44,BCL245,VEGF,telomeric2JSM47,2GKUandaptamerG4TBA)(Zeraatietal.,2018).iMabwasdemonstratedtobeeffectiveintheimmunofluorescencedetectionofi-motifsinmultiplecelllines(MCF7,U2OSandHeLa)simultaneouslyconfirmingthepresenceofi-motifstructuresinhumannucleiandtheirpHandcell-cyclestagedependence(Zeraatietal.,2018).

Antibodyfirstpublishedin:Zeraatietal.I-motifDNAstructuresareformedinthenucleiofhumancells.NatChem.2018Jun;10(6):631-637.doi:10.1038/s41557-018-0046-3.Epub2018Apr23.PMID:29686376Noteonpublication:ThisarticledescribesthegenerationandcharacterisationoftheiMabantibodyfragmentanditsuseinthedetectionofi-motifsinthenucleiofhumancells.