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Alternative Name(s) of Target: G4 DNAImmunogen: Oxy-2 or d(TTTTGGGG)2.
Specificity: The antibody binds to quadruplex DNA with a Kd of 2.64 nM. Binds specifically to only one type of quadruplex DNA, Oxy-2 (d(TTTTGGGG)2).
Application Notes: This antibody binds to quadruplex DNA, which is generated through the association of four guanines bound through Hoogsteen base pairing. G4-forming sequences are associated with immunoglobulin switch regions, promoter sequences, rDNA and telomeric repeats.
Antibody first published in:Henderson et al.Detection of G-quadruplex DNA in mammalian cells.Nucleid Acids 2014; 42(2):860-869PMID:24163102Note on publication:Describes the generation of several G4-DNA-specific monoclonal antibodies and how these can be used for nuclear staining.
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