Cell Line Information:

Designations: C6 Depositors: G Sato Growth Properties: adherent Organism: Rattus norvegicus deposited as Rattus sp. Morphology: fibroblast Source: brain Disease: glioma Cell Type: glial cell; Cellular Products: S-100 protein; produce glyceryl phosphate dehydrogenase in response to glucocorticoids; somatotrophin Applications: transfection host Receptors: glucocorticoid Virus Resistance: poliovirus 3 Comments: The glial cell strain, C6, was cloned from a rat glial tumor induced by N-nitrosomethylurea by Benda et al. S-100 production increases ten fold as cells grow from low density to confluency.

The Transfection Reagent:

C6 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on C6 Cells. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for C6 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of C6 Cell Avalanche™ Transfection Reagent for transfection on C6 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into C6 Cells
• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Suitable for Reverse Transfection
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on C6 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for C6 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this C6 Cell Avalanche Transfection Reagent. FIG. 2. C6 cells were transfected with GFP vector (pEGFP-N3) by using C6 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells:

C6 Cell Avalanche™ Transfection Reagent (rat brain glioma cell) can also be used on the following cells with high transfection efficiencies. U-87 MG Cell NIH/3T3 Cell 3T3-L1 Cell BALB/3T3 clone 3T3-Swiss albino Cell 786-O Cell Caki-1 Cell MDCK Cell 293 Cell 293T/17 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

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