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Exfection™ plasmid LE (Low Endotoxin) and EF(Endotoxin-Free) provide simple and fast method for the purification of plasmid DNA with low endotoxin contaminants.Endotoxins (also known as lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli. It is a common contaminant in plasmid preparations and can significantly reduce transfection efficiencies, if not removed during DNA preparations. These kits use two methods for the removal of endotoxins: advanced phase
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| GA-111-102 | GeneAll Exfection Plasmid LE | 200 mini preps | £164.00 | Quantity | Add to Order |
| GA-111-110 | GeneAll Exfection Plasmid LE | 10 prep sample (1 per customer) | £0.00 | Quantity | Add to Order |
| GA-111-150 | GeneAll Exfection Plasmid LE | 50 mini preps | £75.00 | Quantity | Add to Order |
| GA-111-201 | GeneAll Exfection Plasmid LE | 100 midi prep | £640.00 | Quantity | Add to Order |
| GA-111-226 | GeneAll Exfection Plasmid LE | 26 midi preps | £204.00 | Quantity | Add to Order |
| GA-111-701 | Spin Column for Exfection LE/EF Midi | 25 each | £80.00 | Quantity | Add to Order |
| GA-111-901 | Buffer P1 | 60 ml | £19.60 | Quantity | Add to Order |
| GA-111-903 | Buffer P1 | 100 ml | £22.00 | Quantity | Add to Order |
| GA-111-911 | Buffer P2 | 60 ml | £20.80 | Quantity | Add to Order |
| GA-111-913 | Buffer P2 | 100 ml | £23.20 | Quantity | Add to Order |
| GA-111-921 | Buffer G3 | 90 ml | £32.80 | Quantity | Add to Order |
| GA-111-922 | Buffer G3 | 110 ml | £38.80 | Quantity | Add to Order |
| GA-111-933 | Buffer EW1 | 150ml | £40.00 | Quantity | Add to Order |
| GA-111-935 | Buffer EW1 | 300 ml | £63.00 | Quantity | Add to Order |
| GA-111-944 | Buffer EW2 | 250 ml | £8.00 | Quantity | Add to Order |
| GA-111-946 | Buffer EW2 | 400 ml | £13.00 | Quantity | Add to Order |
| GA-111-952 | Buffer EF | 60 ml | £17.20 | Quantity | Add to Order |
| GA-111-991 | Spin Column for Exfection LE mini | 100 each | £40.00 | Quantity | Add to Order |
| GA-111-992 | Spin Column for Exfection LE mini | 200 each | £66.00 | Quantity | Add to Order |
Exfection™ plasmid LE (Low Endotoxin) and EF(Endotoxin-Free) provide simple and fast method for the purification of plasmid DNA with low endotoxin contaminants.Endotoxins (also known as lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli. It is a common contaminant in plasmid preparations and can significantly reduce transfection efficiencies, if not removed during DNA preparations. These kits use two methods for the removal of endotoxins: advanced phase
Description
ExfectionTM plasmid LE (Low Endotoxin) and EF(Endotoxin-Free) provide simple and fast method for thepurification of plasmid DNA with low endotoxin contaminants.Endotoxins (also known as lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli.It is a common contaminant in plasmid preparations and can significantly reduce transfection efficiencies, if not removed during DNA preparations. These kits use two methods for the removal of endotoxins: advanced phase separation and endotoxin removal washing. Endotoxin levels can be reduced to 0.1 EU/ug with ExfectionTM EF and to 10 EU/ug with ExfectionTM LE. Prepared plasmid DNA can be used for the transfection of most of cell lines in addition to most of molecular biological applications.
Features and Benefits
¦ Spin column format based on glassfiber membrane¦ Convenient clearing of lysate with EzClearTM filter (Midi)¦ High plasmid recoveries with high purity¦ Fast preparation time and simple procedure¦ High transfection efficiency in most cell-lines¦ No need of additional materials¦ No use of organic solvents
Procedure
Specification
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Exfection™ plasmid LE (Low Endotoxin) and EF(Endotoxin-Free) provide simple and fast method for the purification of plasmid DNA with low endotoxin contaminants.Endotoxins (also known as lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli. It is a common contaminant in plasmid preparations and can significantly reduce transfection efficiencies, if not removed during DNA preparations. These kits use two methods for the removal of endotoxins: advanced phase
Click here fro protocol
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Exfection™ plasmid LE (Low Endotoxin) and EF(Endotoxin-Free) provide simple and fast method for the purification of plasmid DNA with low endotoxin contaminants.Endotoxins (also known as lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli. It is a common contaminant in plasmid preparations and can significantly reduce transfection efficiencies, if not removed during DNA preparations. These kits use two methods for the removal of endotoxins: advanced phase
List of references here
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn
