Cell LineInformation

Designations: VCaP Organism: Homo sapiens (human) Tissue: prostate cancer Age: 59 years Gender: male Ethnicity: Caucasian Doubling Time: approximately 53 hours Morphology: epithelial Growth Properties: adherent Amelogenin: XY ( male) Tumorigenicity: yes; in nude and SCID mice Antigen expression: positive for Cytokeratin-18, prostate specific antigen (PSA), prostatic acid phosphatase (PAP), Rb protein and p53 protein. Depositor: K.Pienta This line was established in 1997 from a vertebral bone metastasis from a patient with hormone refractory prostate cancer. It is androgen sensitive in vitro and in vivo.

The Transfection Reagent:

VCaP Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on VCaP Cells. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for VCaP Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of VCaP Cell Avalanche™ Transfection Reagent for transfection on VCaP Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into VCaP Cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Deliver single or multiple plasmids
• Simplest and straightforward protocols
• Compatible with serum
• Suitable for Reverse Transfection
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on PC-3 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for PC-3 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on VCaP cells by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as VCaP Cell Avalanche Transfection Reagent. FIG. 2. VCaP cells were transfected with GFP vector (pEGFP-N3) by using VCaP Cell Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells:

VCaP Cell Avalanche™ Transfection Reagent (human prostate carcinoma cell) can also be used on the following cells with high transfection efficiencies. DU 145 Cell 22Rv1 Cell PC-3 Cell Vero Cell 293 Cell 293T/17 Cell Calu-3 Cell Caki-1 Cell MDCK Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

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