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| Product name | Polyclonal Anti- ACTB |
| Catalog No. | 2107661001 |
| Size | 100μg/vial |
| Form | lyophilized |
| Ig type | rabbit IgG |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminal |
| of human β-Actin, identical to the related rat and mouse | |
| sequences. | |
| Purification | Immunogen affinity purified |
| Reconstitution | 0.2ml of distilled water will yield a concentration of 500μg/ml. |
| Application |
|
| Concentration | Tested Species | Concluded Species | Antigen Retrieval |
| WB | 0.1-0.5μg/ml | Hu,Rat | Ms | - |
Store at -20°C for one year. After reconstitution, store at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
1. Gunning, P., Ponte, P., Okayama, H., Engel, J., Blau, H., Kedes, L. Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed. Molec. Cell. Biol. 3: 787-795, 1983.
2. Karakozova, M., Kozak, M., Wong, C. C. L., Bailey, A. O., Yates, J. R, III, Mogilner, A., Zebroski, H., Kashina, A. Arginylation of beta-actin regulates actin cytoskeleton and cell motility. Science 313: 192-196, 2006.
3. Ueyama, H., Inazawa, J., Nishino, H., Ohkubo, I., Miwa, T. FISH localization of human cytoplasmic actin genes ACTB to 7p22 and ACTG1 to 17q25 and characterization of related pseudogenes.Cytogenet. Cell Genet. 74: 221-224, 1996.