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Everest Biotech/Monoclonal Anti-Bcl-2 100μg/vial/2005081001/100μg/vial


Clone
BL-2
Size
100μg/vial
Form
lyophilized
Igtype
mouseIgG1
Specificity
Nocrossreactivitywithotherproteins.
Species
Human
Immunogen
Syntheticpeptidecorrespondingtoresidues41-54oftheBcl-2protein,conjugated
tothyroglobulin.
Contents
Mouseascitesfluid,1.2%sodiumacetate,2mgBSA,with0.01mgNaN3as
preservative.

Westernblot
At1-2μg/mlwiththeappropriatesystemtodetectBCL-2incellsandtissues.
Immunohistochemistry
At0.4-1μg/mltodetectBCL-2informalinfixedandparaffinembeddedtissues.
(Paraffin-embeddedSection)
Immunohistochemistry
At0.4-1μg/mltodetectBCL-2informalin/acetonefixedtissues.
(FrozenSection)
Immunocytochemistry
Suitable

Otherapplicationshavenotbeentested.
Optimaldilutionsshouldbedeterminedbyendusers.

Reconstitution:1.2%sodiumacetateorneutralPBS.If1mlofPBSisused,theantibodyconcentrationwillbe100μg/ml.
Storage:At-20˚Cforoneyear.Afterreconstitution,at4˚Cforonemonth.Itcanalsobealiquottedandstoredfrozenat-20˚Cforalongertime.Avoidrepeatedfreezingandthawing.

1.Ngan,B.-Y.;Chen-Levy,Z.;Weiss,L.M.;Warnke,R.A.;Cleary,M.L.:Expressioninnon-Hodgkin"slymphomaoftheBCL-2proteinassociatedwiththet(14;18)chromosomaltranslocation.NewEng.J.Med.318:1638-1644,1988.
2.Hockenbery,D.;Nunez,G.;Milliman,C.;Schreiber,R.D.;Korsmeyer,S.J.:Bcl-2isaninnermitochondrialmembraneproteinthatblocksprogrammedcelldeath.Nature348:334-336,1990.

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