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Everest Biotech/Monoclonal Anti-γ-Tubulin 100μg/vial/2006021001/100μg/vial

Clone
IMD-20
Size
100μg/vial
Form
lyophilized
Igtype
mouseIgG1
Specificity
Nocrossreactivitywithotherproteins.
Species
Human,mouse,rat
Immunogen
Syntheticγ-tubulinpeptide(aminoacids38-53),conjugatedtoKLH.
Contents
Mouseascitesfluid,1.2%sodiumacetate,2mgBSA,with0.01mgNaN3as
preservative.

Westernblot
At0.25-0.5μg/mlwiththeappropriatesystemtodetectγ-Tubulinincellsand
tissues.
Immunocytochemistry
Suitable

Otherapplicationshavenotbeentested.
Optimaldilutionsshouldbedeterminedbyendusers.

Reconstitution:1.2%sodiumacetateorneutralPBS.If1mlofPBSisused,theantibodyconcentrationwillbe100μg/ml.
Storage:At-20˚Cforoneyear.Afterreconstitution,at4˚Cforonemonth.Itcanalsobealiquottedandstoredfrozenat-20˚Cforalongertime.Avoidrepeatedfreezingandthawing.

1.Rommens,J.M.;DuRocher,F.;McArthur,J.;Tonin,P.;LeBlanc,J.-F.;Allen,T.;Samson,C.;Ferri,L.;Narod,S.;Morgan,K.;Simard,J.:GenerationofatranscriptionmapattheHSD17BlocuscentromerictoBRCA1at17q21.Genomics28:530-542,1995.

2.Zheng,Y.;Jung,M.K.;Oakley,B.R.:Gamma-tubulinispresentinDrosophilamelanogasterandHomosapiensandisassociatedwiththecentrosome.Cell65:817-823,1991.

3.Stearns,T.;Evans,L.;Kirschner,M.:Gamma-tubulinisahighlyconservedcomponentofthecentrosome.Cell65:825-836,1991.

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