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Everest Biotech/Human Betacellulin/BTC ELISA Kit 1 plate/2903870015/1 plate188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Everest Biotech/Human Betacellulin/BTC ELISA Kit 1 plate/2903870015/1 plate


For quantitative detection of human Betacellulin in cell culture supernates, serum and plasma(heparin, EDTA).
Typical Data Obtained from Human Betacellulin
(TMB reaction incubate at 37°C for 25 min)
Concentration(pg/ml)
0.0
15.6
31.2
62.5
125
250
500
1000
O.D.
0.073
0.086
0.112
0.179
0.285
0.540
0.961
1.650
Typical Human Betacellulin ELISA Kit Standard Curve
This standard curve was generated at Eton for demonstration purpose only. A standard curve must be run with each assay.


Range 15.6pg/ml-1000pg/ml

Sensitivity < 10pg/ml
Specificity Natural and recombinant human Betacellulin
Cross-reactivity No detectable cross-reactivity with other relevant proteins
Storage
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plateto assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separateassays to assess inter-assay precision.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
16
16
16
24
24
24
Mean(pg/ml)
46
119
725
47
124
736
Standard deviation
2.35
6.31
45.68
2.73
7.69
58.14
CV(%)
5.1
5.3
6.3
5.8
6.2
7.9
Principle
Eton’s human Betacellulin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Betacellulin has been precoated onto 96-well plates. Standards(E.coli, D32-Y111) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Betacellulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Betacellulin amount of sample captured in plate.
Kit Components
Description
Quantity
96-well plate precoated with anti- human Betacellulin antibody
1
Lyophilized recombinant human Betacellulin standard
10ng/tube×2
Biotinylated anti- human Betacellulin antibody
130μl(dilution 1:100)
Avidin-Biotin-Peroxidase Complex (ABC)
130μl(dilution 1:100)
Sample diluent buffer
30 ml
Antibody diluent buffer
12ml
ABC diluent buffer
12ml
TMB color developing agent
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