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HansaBioMed/Peroxidase Activity Assay Kit (100 reactions)/HBM-K772-100/1 Ea

PeroxidaseActivityColorimetric/FluorometricAssayKit.

HighlySensitiveAssay,HTS

 

KitSummary:

•Detectionmethod-Absorbance(570nm)orFluorescence(Ex/Em=535/587)

•Speciesreactivity-Mammalian

•Application-Thekithasadetectionlimitof0.1mUperwellviacolorimetricor0.01mUperwellviafluorometricmethod,basedonourunitdefinition.

 

Features&Benefits:

•Simpleprocedure

•Fastandconvenient

•Theassayissimple,direct,highlysensitiveandhigh-throughputadaptable.

 

KitComponents:

•AssayBuffer

•OxiRed™Probe(inDMSO)

•H2O2Substrate(0.88M)

•HRPPositiveControl

 

Description:

Peroxidases(ECnumber1.11.1.x)arealargefamilyofenzymesthattypicallycatalyzeareactionoftheform:ROOR"+electrondonor(2e-)+2H+→ROH+R"OH.Formanyoftheseenzymestheoptimalsubstrateishydrogenperoxide,butothersaremoreactivewithorganichydroperoxidessuchaslipidperoxides.Peroxidasescancontainahemecofactorintheiractivesites,oralternatelyredox-activecysteineorselenocysteineresidues.HansABIoMed’sPeroxidaseAssayKitprovidesaconvenientcolorimetricandfluorometricmeanstomeasuretheperoxidaseactivityinBIOLOGicalsamples.InthepresenceofPeroxidase,theOxiRedProbereactswithH2O2ina1:1stoichiometrytoproducethered-fluorescentoxidationproduct,resorufin.Theresorufinisquantifiedbycolorimetric(lmax=570nm)orfluorometricmethods(Ex/Em=535/587nm).Theassayissimple,direct,highlysensitiveandhighthroughput-ready.Thedetectionlimitis0.1mUperwellviacolorimetricor0.01mUperwellviafluorometricmethod,basedonourunitdefinition.

 

StorageConditions:-20°C.

 

ShippingConditions:gelpack.

 

USAGE:ForResearchUseOnly!NotForUseinHumans.

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