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HansaBioMed/HAT Fluorometric Kit (100 reactions)/HBM-K334-100/1 Ea

HATActivityFluorometricAssayKit.

SensitiveassaytomeasureHATactivity

 

KitSummary:

•Detectionmethod-Fluorescence(Ex/Em=535/587nm)

•Speciesreactivity-N/A

•Applications:

-MeasurementofHATactivityinNuclearExtracts

-MeasurementofHATactivityofpurifiedenzymepreparations.

 

SampleType:

•Nuclearextractsfromcellsandtissue

•Recombinantenzyme

 

Features&Benefits:

•Simple,rapid&convenient

•TheassaycandetectHATActivityaslowas6mUinavarietyofsamples

 

KitComponents:

•HATAssayBuffer

•AcetylCoA(Lyophilized)

•H3Peptide(Lyophilized)

•SubstrateMix(Lyophilized)

•Developer

•PicoProbe™

•CoAStandard(Lyophilized)

•PositiveControl(HeLaNuclearExtract)

 

Description:

HistoneAcetyltransferases(HATs)areenzymesthatacetylatehistonesubstratesresultinginimportantregulatoryeffectsonchromatinstructureandassembly,andgenetranscription.ModificationsoftheseproteinsbyHATsplayanimportantroleinthecontrolofgeneexpression,andtheirdysregulationhasbeenlinkedtocancer,neurodegeneration,andotherdiseases.HansABIoMed"sHATActivityAssayKitutilizesAcetylCoAandH3histonepeptideassubstrates.Inthisassay,HATenzymecatalyzesthetransferofacetylgroupsfromAcetyl-CoAtothehistonepeptide,therebygeneratingtwoproducts-acetylatedpeptideandCoA-SH.TheCoA-SHreactswiththedevelopertogenerateaproductthatisdetectedfluorometricallyatEx/Em=535/587nm.TheassaycandetectHATactivityaslowas6mUinavarietyofsamples.

 

StorageConditions:-80°C.

 

ShippingConditions: dryice.

 

USAGE:ForResearchUseOnly!NotForUseinHumans.

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