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Innova Biosciences/Latex conjugation kit – 400nm Black/1004-0040/4 reaction mini kit

OurLatexConjugationKitallowsantibodiesorproteinstobeconjugatedtoourhighquality400nmblacklatexbeadsquicklyandeasily.ThelatexbeadsaredesignedforeaseofuseandhavemuchimprovedhandlingcomparedwithtrADItionallatex.Thishasbeenachievedbyspeciallytreatingtheparticles.Theconjugationiscovalent,andrequireslessantibodythantraditionalpassiveandcovalentlatexconjugation.

The400nmblacklatexbeadsinthiskitarefreezedried.Theconjugationreactionisinitiatedsimplybyreconstitutingthedrymixturewithyourantibody,whichbecomesattached(vialysineresidues)tothespeciallytreatedsurface.

LATEXconjugationprocedure

Latex conjugation kit - 400nm Black

  • 30secondstosetuptheconjugation
  • 3minuteshands-ontimefortheconjugationprocedure
  • Conjugateisreadytousewithin35minutes.YousimplyPipettethebiomoleculeintoavialcontainingthelatexnanoparticles,thencentrifugetobufferexchange.

Pleaserefertoour customservices forconjugationservicesor getincontact withustoknowmoreaboutthisproduct.

Figure1.LateralFlowAssayofourspecially-treatedlatexbeadsconjugatedtoamonoclonalanti-CRPantibody(mAb1)titratedagainstCRP.2ng/mlCRPcaneasilybedetected.

Figure2.LateralFlowAssayofourspecially-treatedlatexconjugatedtothemonoclonalanti-CRPantibody(mAb2)titratedagainstCRPinbufferorCRPspikedCRP-depletedserum(100%serum).Thelatexbehavessimilarlyinserumandbuffer,andnoaggregationornon-specificbindingisseen.

Figure3.MultiplexedLatex.Thethreecoloursoflatexeachconjugatedtoadifferentantibodyorproteinandformingalineeitherbyadirectbindingevent(redandblacklatex)orasandwichassaybindingevent(bluelatex).Thethreecoloursoflatexdemonstratenoaggregationorbackgroundstaining.

  • Towardsapoint-of-carestriptesttodiagnosesicklecellanemia- PLoSOne(2017)

    Viewreference

  • Two-ColorLateralFlowAssayforMultiplexDetectionofCausativeAgentsBehindAcuteFebrileIllnesses - AnalyticalChemistry(2016)

    Viewreference

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