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Innova Biosciences/Lightning-Link® APC/Cy7/765-0010/3 reactions each up to100ug

TheInnovaBiosciencesLightning-Link^®APC/Cy7antibodylabelingkitenablesthedirectconjugationofantibodies,proteinsandpeptides,oranyotherbiomoleculewithanavailableaminegroup,toAPC/Cy7withonly30secondshands-ontimeandwith100%recoveryofmaterials.Thetechnologyisfullyscalablefrom10ugto1gormoreandstringentlyQCtestedforconsistenthighqualityandexcellentbatch-to-batchreproducibility.

Lightning-Link_process_diagram

APC/Cy7isatandemconjugate.TheAPCisexcitedat652nmandfunctionsasanenergydonorfortheCy7.EnergyistransferredfromtheAPCtotheCy7viaFRET(FluorescenceResonanceEnergyTransfer).TheCy7emitstheenergyreceivedfromtheAPCintheformoflongwavelengthlightat790nm.

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FlowCytometrydata–APC/Cy7

Mouseanti-humanCD8wasconjugatedwithAPC-Cy7usinganInnovaBiosciencesLightning-Link®kit.Theconjugatedantibodywasthenusedtostainhumanperipheralbloodlymphocytes,followedbyanalysiswithflowcytometry.(Blueline–negativecontrol;redline–positivestaining).

Predictionofbreastcancerriskbasedonflow-variantanalysisofcirculatingperipheralbloodBcells - GenetMed.(2017)
Viewreference
AssociationofTatwithPromotersofPTENandPP2ASubunitsIsKeytoTranscriptionalActivationofApoptoticPathwaysinHIV-InfectedCD4+TCells-PLoSPathogens(2010)
Viewreference
High-mobilitygroupbox1protein(HMGB1)neutralizationamelioratesexperimentalautoimmuneencephalomyelitis-JournalofAutoimmunity(2013)
Viewreference
GAS6ReceptorStatusIsAssociatedwithDormancyandBoneMetastaticTumorFormation-PLoSOne(2013)
Viewreference
RegulationofT-PlastinExpressionbyPromoterHypomethylationinPrimaryCutaneousT-CellLymphoma-JournalofInvestigativeDermatology(2013)
Viewreference
CharacterizationofOligodendroglialPopulationsinMouseDemyelinatingDiseaseUsingFlowCytometry:CluesforMSPathogenesis-PLoSOne(2014)
Viewreference
Designofamulti-centerimmunophenotypinganalysisofperipheralblood,sputumandbronchoalveolarlavagefluidintheSubpopulationsandIntermediateOutcomeMeasuresinCOPDStudy(SPIROMICS)-JournalofTranslationalMedicine(2015)
Viewreference

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Innova Biosciences/Lightning-Link® APC/Cy7/765-0010/3 reactions each up to100ug

Predictionofbreastcancerriskbasedonflow-variantanalysisofcirculatingperipheralbloodBcells - GenetMed.(2017)

AssociationofTatwithPromotersofPTENandPP2ASubunitsIsKeytoTranscriptionalActivationofApoptoticPathwaysinHIV-InfectedCD4+TCells-PLoSPathogens(2010)

High-mobilitygroupbox1protein(HMGB1)neutralizationamelioratesexperimentalautoimmuneencephalomyelitis-JournalofAutoimmunity(2013)

GAS6ReceptorStatusIsAssociatedwithDormancyandBoneMetastaticTumorFormation-PLoSOne(2013)

RegulationofT-PlastinExpressionbyPromoterHypomethylationinPrimaryCutaneousT-CellLymphoma-JournalofInvestigativeDermatology(2013)

CharacterizationofOligodendroglialPopulationsinMouseDemyelinatingDiseaseUsingFlowCytometry:CluesforMSPathogenesis-PLoSOne(2014)

Designofamulti-centerimmunophenotypinganalysisofperipheralblood,sputumandbronchoalveolarlavagefluidintheSubpopulationsandIntermediateOutcomeMeasuresinCOPDStudy(SPIROMICS)-JournalofTranslationalMedicine(2015)