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Neuromics/Prominin 2/MO15051100/100 ug

Type:MouseIgG

 

Applications:ICC;FC

E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssays

SpeciesReactivity:H

B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;All

Format:ProteinGPurified-liquid

 

Immunogen:HybridomaresultingfromthefusionofamousewithBcellsobtainedfromamouseimmunizedwithProminin2-transfectedNS0mousemyelomacells(clone#:244029).

 

Picture

Prominin2isapentaspanmembraneglycoproteinpredominantlyexpressedinneuroepithelialcells,hematopoieticstemcellsandepithelialcellsoftheadultkidneyanddigestivetract.

Images:FluorescencemicroscopyanalysisofexpressionofPROM2(redsignal),aK-Rasdependencysignaturegene,inK-Ras-dependent(H358andYAPC)andKRas-independent(PA-TU-8988TandA549)celllines.GreensignalcorrespondstoFactin,asrevealedbyphalloidinstaining.Scalebar=15μM.CancerCell.2009June2;15(6):489–500.doi:10.1016/j.ccr.2009.03.022.

Prominin2expressionisdown-regulatedinaggressiveprostatecancercelllinesandtransienttransfectionofPROML2expressionvectorshasbeenshowntoinduceapoptosisinculturedprostatecancercells,suggestingatumorsuppressiveroleforProminin2.Prominin2expressionislikelytobeinvolvedingrowthsuppressionintheprostate,anddown-regulationofProminin2maydisruptnormalprostatichomeostasisandleadtouncontrolledprostaticgrowth.

Prominin2:CustomerPublications


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