
Humanglutamatereceptor4(GRIA4)isanewmemberofthefamilyofionotropicglutamatereceptorswhicharethepredominantexcitatoryneurotransmitterreceptorsinthemammalianbrain.BindingstudiesshowedthathumanGluR4transfectedintosimiankidneycells(COS-1)exhibitshighspecificbindingfor[3H](RS)-alpha-amino-3-hydroxy-5-methylisoxazole-4-propionicacid([3H]AMPA)butnot[3H]kainate.IonsubstitutionexperimentsindicatethathGluR4receptor-linkedionchannelsintheirhomomericstatearepermeabletobothCA2+andNa+ions.ImmunoprecipitationstudiessuggestthatGRIA1existsinsituintheformofapentamer.
Image:GluR4stainingofhumanbraintissue,cortex(formalin-fixedparaffin-embedded)at5ug/mlfollowedbybiotinylated horseanti-goatIgGsecondaryantibody, alkalinephosphatase-streptavidinandchromogen.Protocolondata-sheet.
Nociceptors,involvedprimarilyinthemediationofneuropathicpain,maybepresynapticallymodulatedbyGluR4-containingAMPAreceptors.