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Neuromics/cKit/CD117/RA14132/100 ul

Type:RabbitIgG

 

Applications:IF;IHC;FC

E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssays

SpeciesReactivity:H;M;R

B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;All

Format:AffinityPurified-liquid

 

Immunogen:TASSSQPLLVHDDV

 

Picture

c-Kitistransmembranetyrosinekinaseencodedbythec-Kitprotooncogene.c-Kitactstoregulateavarietyofbiological processesincludingcellproliferation,apoptosis,chemotaxisandadhesion.
Itplaysacriticalroleinactivationandgrowthinanumberofcelltypessuchashematopoieticstemcells,mastcells,melanocytesandgermcells.

Mutationsinc-Kithavebeenfoundtobeimportantfortumorgrowthandprogressioninavarietyofcancersincludingmastcelldiseases,gastrointestinalstromaltumor,acutemyeloidleukemia,Ewingsarcomaandlungcancer.Phosphorylationattyrosine721ofc-KitallowsbindingandactivationofPI3kinase.

Image:c-KITstainingofratskin(epidermis).c-KITdetectionwasdoneusinganti-rabbitCy3conjugatedantibodies(redcolor).DAPIwasusedtocounterstaincellnuclei(bluecolor).Workingdilution:1:100-1:300. 

c-Kit:CustomerPublications


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