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Neuromics/Neurofilament NFM/CH22106/100 ul

Type:ChickenIgY

 

Applications:ICC;IHC;WB

E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssays

SpeciesReactivity:Ch;H;M;R

B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;All

Format:liquid

 

Immunogen:C-terminalextensionofratNF-M,theso-calledKEsegment,wasexpressedinbacteriaandpurified.

 

Picture

Neurofilamentscanbedefinedastheintermediateor10nmfilamentsfoundinspecificallyinneuronalcells.Intheelectronmicroscopeneurofilamentsappearsas10nmdiameterfibresofindeterminatelengthwhichgenerallyhavefinewispyprotrusionsfromtheirsides.Theyarefoundparticularlyabundantlyinaxonsoflargeprojectionneurons.AntibodiestothevariousneurofilamentsubunitsareveryusefulcelltypeMarkerssincetheproteinsareamongthemostabundantofthenervoussystem,areexpressedonlyinneurons,andarebiochemicallyverystable.

Viewofmixedneuron/glialculturesstainedwithNeurofilament-M(red).TheNF-Mproteinisassembledintoneurofilamentswhicharefoundthroughouttheaxons,dendritesandperikaryaofthesecells.Protocolandatasheet.

Neurofilament-M (NF-M)is themediumormiddlerunsatabout145-160kDa.


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