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| ProductDetails | |
|---|---|
| Synonyms | MultimericTNF-α™;ACRP30headless:TNF-α;ACRP30headless:TNFSF2;ACRP30headless:TumorNecrosisFactor-α |
| ProductType | Protein |
| Properties | |
| Source/Host | HEK293cells |
| Sequence | TheextracellulardomainofhumanTNF-α(aa85-233)isfusedattheN-terminustomouseACRP30headless(aa18-111)andaFLAG®-tag. |
| Crossreactivity | Human Mouse |
| Specificity | BindstohumanandmouseTNF-R1andTNF-R2. |
| BiologicalActivity | ActivateshumanandmouseTNF-R1andTNF-R2.Inducescelldeathataconcentrationrangeof0.01-10ng/ml(ED50=0.05ng/mlor2x10E7Units/mg;WEHI164cells). |
| MW | ~34kDa(SDS-PAGE) |
| Purity | ≥95%(SDS-PAGE) |
| EndotoxinContent | <0.02EU/μgpurifiedprotein(LALtest;Lonza). |
| Concentration | 0.1mg/mlafterreconstitution. |
| Reconstitution | Reconstitutewith100μlsterilewater. |
| Formulation | Lyophilized.ContainsPBS. |
| OtherProductData | UniProtlinkP01375:TNF-α(human) FLAGisaregisteredtrademarkofSigma-AldrichCo. |
| ShippingandHandling | |
| Shipping | BLUEICE |
| ShortTermStorage | +4°C |
| LongTermStorage | -20°C |
| HandlingAdvice | Afterreconstitution,preparealiquotsandstoreat-20°C. Avoidfreeze/thawcycles. PBScontainingatleast0.1%BSAshouldbeusedforfurtherdilutions. |
| Use/Stability | Stableforatleast6monthsafterreceiptwhenstoredat-20°C. |
| Documents | |
| MSDS |  DownloadPDF |
| ProductSpecificationSheet | |
| Datasheet | DownloadPDF |