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| Product Details | |
|---|---|
| Synonyms | OB Gene Product; Obese Factor |
| Product Type | Polyclonal Antibody |
| Properties | |
| Source/Host | Rabbit |
| Immunogen/Antigen | Recombinant mouse leptin. |
| Label/Conjugates | Biotin |
| Application |
ELISA: (direct and indirect: 1:5’000-1:10’000) Immunoprecipitation: (1-5 μl per sample in a final reaction volume of 0.5-1 ml) Western Blot: (1:5’000-1:10’000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application. |
| Crossreactivity | Mouse |
| Specificity | Recognizes mouse leptin. Weakly cross-reacts with human and rat leptin. Detects a band of ~16kDa by Western blot. |
| Purity Detail | Protein A-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 6 months after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
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