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Adipogen/ODN 2395 (Type C) Endotoxin-free (sterile)/IAX-200-007-3001/3 x 1 mg

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ProductDetails
Synonyms	CpGODN:CType(species:humanandrodent)
ProductType	Chemical
Properties
MW	7,068g/mol
Sequence	5"-tcgtcgttttcggcgcgcgccg-3"(lowercaseletters:phosphorothioatelinkage:nucleaseresistant).
Reconstitution	Fora100μMstocksolution,dissolvetotalvialcontentin1410μl(1mgsize)or141μl(100μgsize)sterileendotoxin-freewaterorPBS.
Formulation	Lyophilized.Sterile.
BIOLOGicalActivity	Optimalconcentrationisdependentuponcelltype,species,desiredactivationandanalysis:invitro0.1-1μM(~0.7-7.0μg/ml);invivo50-250μginrodentanimalmodels. Negativecontrol:Neutral-ODN(Cat.No.:IAX-200-202).
Crossreactivity	Human Mouse Rat
Specificity	Human,Mouse,Rat
EndotoxinContent	<0.002eu g="">
Declaration	ManufacturedbyInnaxon.
OtherProductData	Contains:100μgsizeincludes1.5mlddWaterEndotoxin-free(sterile)(Cat.No.:IAX-900-002-LD15).1mgand3x1mgsizeseachinclude10mlddWaterEndotoxin-free(sterile)(Cat.No.:IAX-900-002-L010). ReconstitutionNote:Add50%ofsolventandletdissolvefor10min.Addremaining50%ofthesolventandmixthoroughly.Moderatewarmingmayaiddissolving. ClickhereforOriginalManufacturerProductDatasheet:Ourproductdescriptionmaydifferslightlyfromtheoriginalmanufacturersproductdatasheet.
ShippingandHandling
Shipping	AMBIENT
ShortTermStorage	+4°C
LongTermStorage	+4°C
HandlingAdvice	Avoidfreeze/thawcycles. Afterreconstitution,preparealiquotsandkeepaqueousstocksolutionsfor1dayat4°Corstoreat-20°C(shelf-life6months).
Use/StABIlity	Stableforatleast2yearsafterreceiptwhenstoredat+4°C.
Documents
MSDS	DownloadPDF
ProductSpecificationSheet
Datasheet	DownloadPDF

UnmethylatedCGdinucleotideswithinparticularsequencecontextsareresponsIBLefortheimmunostimulatoryactivityofbacterialDNA.Syntheticoligonucleotides(ODN)thatcontainsuchCpGmotifs(CpGODNs)mimicmicrobialDNA.TheinnateimmunesystemofvertebrateshastheabilitytorecognizeCpGmotifsinmicrobialDNAviatheToll-likereceptor(TLR)9iftheCpGODNwerefreeofadditionalimmunestimulatorycontaminantsoftenpresentinsyntheticcommercialCpGODNpreparationsdesignedformolecularbiologyapplications(i.e.PCR).GiventhathighqualityCpGODNswereused[i.e.endotoxin-free],acloselinkhasbeenestablishedbetweentheexpressionofTLR9oncertainimmunecellsubsetsandthemodulationoftheimmunesystembyCpGDNA.DifferenttypesofCpGODNswereidentifiedbasedontheirdifferingbiologicaleffectsondifferentcelltypes:ODNTypeAisapotentinducerofIFN-αinhumanPDC,(i.e.ODN1585or2216)leADIngtoantigenpresentingcell(APC)maturation,whereasODNTypeB(i.e.ODN2006orODN1668/ODN1826)isaweakinducerofIFN-αbutratherstimulatesIL-8productionandincreasingcostimulatoryandAg-presentingmoleculesandtriggersproliferationofB-cellsandIgMandIL-6production.AthirdtypeofCpGODNhasbeenidentified,termedODNTypeC,withbothhighinductionofINF-αinPDCandactivationofB-cells.ThesequenceofCpGTypeC(alsocalledK)(i.e.ODN2395orM362)combineselementsofbothTypeAandTypeBandcontainsacentralpalindromicsequencewithCGdinucleotides,acharacteristicfeatureofTypeA,andaTCGTCGmotifatthe5"end,presentinTypeBCpGODNs.AlthoughtheCpGmotifsarethoughttodifferbetweenmiceandhumans,inbothspeciestherecognitionofCpGODNsismediatedbyTLR9.TheoptimalCpGmotifinhumansisGTCGTTandGACGTTforthemurinesequence.However,recentevidencesuggeststhatthissequencespecificityisrestrictedtophosphorothioate(PS)-modifiedODNandisnotobservedwhenanaturalphosphodiesterbackboneisused.Inrecentyearssequencerequirements,specificity,signalingpathwaysandkineticsoftheTLR9suppressionbyinhibitoryODNs(iODNs)havebeeninvestigated.

ProductReferences

ComparisonofdifferentCpGoligodeoxynucleotideclassesfortheircapabilitytostimulatehumanNKcells:S.Sivori,etal.;Eur.J.Immunol.36,961(2006)
InnateimmuneresponsesinducedbyclassesofCpGoligodeoxynucleotidesinovinelymphnodeandbloodmononuclearcells:J.S.Booth,etal.;Vet.Immunol.Immunopathol.115,24(2007)
ImpactofclassA,BandCCpG-oligodeoxynucleotidesoninvitroactivationofinnateimmunecellsinhumanimmunodeficiencyvirus-1infectedindividuals:J.A.Martinson,etal.;Immunology120,526(2007)
ThreeCpGoligodeoxynucleotideclassesdifferentiallyenhanceantigen-specifichumoralandcellularimmuneresponsesinmice:Y.Liu,etal.;Vaccine29,5778(2011)
Toll-likereceptorandIL-12signalingcontrolsusceptibilitytocontacthypersensitivity:S.F.Martin,etal.;J.Exp.Med.205,2151(2008)

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Adipogen/ODN 2395 (Type C) Endotoxin-free (sterile)/IAX-200-007-3001/3 x 1 mg