Adipogen/ProSUMO3 (human) (rec.) (His)/AG40T0343C500/500 µg

SmallUbiquitin-likeModifier3(SUMO3),alsoknownasSMT3A,issynthesizedasa103aminoacid(aa),propeptidewithapredicted11.5kDa.SUMO3containsatwoaaC-terminalprosegment.HumanSUMO3shares83%sequenceidentitywithmouseSUMO3.SUMO3alsohashighaasequencehomologytoSUMO2andSUMO4,87%and75%,respectively.SUMO3sharesonly47%sequenceidentitywithSUMO1.SUMOsareafamilyofsmall,relatedproteinsthatcanbeenzymaticallyattachedtoatargetproteinbyapost-translationalmodificationprocesstermedSUMOylation.AllSUMOproteinsshareaconservedubiquitindomainandaC-terminaldiglycinecleavage/attachmentsite.Followingprosegmentcleavage,theC-terminalglycineresidueofSUMO3isenzymaticallyattachedtoalysineresidueonatargetprotein.Inhumans,SUMO3isconjugatedtoavarietyofmoleculesinthepresenceoftheSAE1/UBA2SUMO-activating(E1)enzymeandtheUBE2I/Ubc9SUMO-conjugating(E2)enzyme.Inyeast,theSUMO-activating(E1)enzymeisAos1/Uba2p.BecauseofthehighlevelofsequencehomologymoststudiesreporteffectsofSUMO2/3.Forexample,additionofSUMO2/3wasshowntomodulatethefunctionofARHGAP21,aRhoGAPproteinknowntobeinvolvedincellmigration.OtherreportsindicatethattheconjugationbySUMO2/3,butnotSUMO1,mayrepresentanimportantmechanismtoprotectneuronsduringepisodesofcerebralischemia.However,studiessuggestthatSUMO2/3expressionisregulatedinanisoform-specificmannersinceoxidativestressdownregulatedthetranscriptionofSUMO3,butnotSUMO2.AllSUMOisoformsaretranslatedwithadditionalC-terminalresiduesthathavetoberemovedtogeneratetheactiveprotein.Pro-SUMO-3(103aa)istheinactiveprecursorofSUMO-3(92aa)andisprocessedattheC-terminusbySUMO-3specificproteases(SENPs).TheresultingSUMO-3proteinhastheconservedC-terminalGly-Glyresiduesthatfunctioninactivationandconjugationreactions.