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| ProductDetails | |
|---|---|
| Synonyms | CSF;CSF2;GMCSF;GM-CSF;Molgramostin;Sargramostim;Colony-stimulatingFactor;Granulocyte-macrophageColony-stimulatingFactor |
| ProductType | Protein |
| Properties | |
| Source/Host | E.coli |
| Sequence | HumanGM-CSF(aa18-144)isfusedattheC-terminustoaHis-tag. |
| Crossreactivity | Human |
| BiologicalActivity | MeasuredinacellproliferationassayusingTF-1humanerythroleukemiccells.TheED50forthiseffectistypically0.1-0.6ng/mL. |
| MW | ~16kDa(SDS-PAGE) |
| Purity | ≥95%(SDS-PAGE) |
| EndotoxinContent | <0.1eu g="" protein="" (lal="" test;="" lonza).=""> |
| Reconstitution | Reconstitute10µgvialwith100µlsterilewatertoaconcentrationof0.1mg/ml. Reconstitute50µgvialwith100µlsterilewatertoaconcentrationof0.5mg/ml. Add1XPBStothedesiredproteinconcentration. |
| Formulation | Lyophilizedfrom0.2μm-filteredsolutionin50mMTris-HCl,200mMNaCl,pH8.0. |
| OtherProductData | NCBIreferenceAAI14000.1:GM-CSF(human) |
| Declaration | ManufacturedbyChimerigen. |
| ShippingandHandling | |
| Shipping | BLUEICE |
| ShortTermStorage | +4°C |
| LongTermStorage | -20°C |
| HandlingAdvice | Avoidfreeze/thawcycles. PBScontainingatleast0.1%BSAshouldbeusedforfurtherdilutions. |
| Use/Stability | Stableforatleast1yearafterreceiptwhenstoredat-20°C. Workingaliquotsarestableforupto3monthswhenstoredat-20°C. |
| Documents | |
| MSDS | No |
| ProductSpecificationSheet | |
| Datasheet |  DownloadPDF |