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| ProductDetails | |
|---|---|
| Synonyms | CpGODNTLR9Agonist:BType(species:rodent) |
| ProductType | Chemical |
| Properties | |
| MW | 6,383g/mol |
| Sequence | 5-tccatgacgttcctgatgct-3(lowercaseletters:phosphorothioatelinkage:nucleaseresistant). |
| Reconstitution | Fora100μMstocksolution,dissolvetotalvialcontentin1570μl(1mgsize)or157μl(100μgsize)sterileendotoxin-freewaterorPBS. |
| Formulation | Lyophilized.Sterile. |
| BiologicalActivity | Optimalconcentrationisdependentuponcelltype,species,desiredactivationandanalysis:invitro0.1-1μM(~0.6-6.0μg/ml);invivo50-250μginrodentanimalmodels. |
| Crossreactivity | Mouse Rat |
| Specificity | Mouse,Rat |
| EndotoxinContent | <0.002eu g=""> |
| Declaration | ManufacturedbyInnaxon. |
| OtherProductData | Contains:100μgsizeincludes1.5mlddWaterEndotoxin-free(sterile)(Cat.No.:IAX-900-002-LD15).1mgand3x1mgsizeseachinclude10mlddWaterEndotoxin-free(sterile)(Cat.No.:IAX-900-002-L010). |
| ShippingandHandling | |
| Shipping | AMBIENT |
| ShortTermStorage | +4°C |
| LongTermStorage | +4°C |
| HandlingAdvice | Avoidfreeze/thawcycles. Afterreconstitution,preparealiquotsandkeepaqueousstocksolutionsfor1dayat4°Corstoreat-20°C(shelf-life6months). |
| Use/Stability | Stableforatleast2yearsafterreceiptwhenstoredat+4°C. |
| Documents | |
| MSDS |  DownloadPDF |
| ProductSpecificationSheet | |
| Datasheet | DownloadPDF |
UnmethylatedCGdinucleotideswithinparticularsequencecontextsareresponsiblefortheimmunostimulatoryactivityofbacterialDNA.Syntheticoligonucleotides(ODN)thatcontainsuchCpGmotifs(CpGODNs)mimicmicrobialDNA.TheinnateimmunesystemofvertebrateshastheabilitytorecognizeCpGmotifsinmicrobialDNAviatheToll-likereceptor(TLR)9iftheCpGODNwerefreeofadditionalimmunestimulatorycontaminantsoftenpresentinsyntheticcommercialCpGODNpreparationsdesignedformolecularbiologyapplications(i.e.PCR).GiventhathighqualityCpGODNswereused[i.e.endotoxin-free],acloselinkhasbeenestablishedbetweentheexpressionofTLR9oncertainimmunecellsubsetsandthemodulationoftheimmunesystembyCpGDNA.DifferenttypesofCpGODNswereidentifiedbasedontheirdifferingbiologicaleffectsondifferentcelltypes:ODNTypeAisapotentinducerofIFN-αinhumanPDC,(i.e.ODN1585or2216)leadingtoantigenpresentingcell(APC)maturation,whereasODNTypeB(i.e.ODN2006orODN1668/ODN1826)isaweakinducerofIFN-αbutratherstimulatesIL-8productionandincreasingcostimulatoryandAg-presentingmoleculesandtriggersproliferationofB-cellsandIgMandIL-6production.AthirdtypeofCpGODNhasbeenidentified,termedODNTypeC,withbothhighinductionofINF-αinPDCandactivationofB-cells.ThesequenceofCpGTypeC(alsocalledK)(i.e.ODN2395orM362)combineselementsofbothTypeAandTypeBandcontainsacentralpalindromicsequencewithCGdinucleotides,acharacteristicfeatureofTypeA,andaTCGTCGmotifatthe5end,presentinTypeBCpGODNs.AlthoughtheCpGmotifsarethoughttodifferbetweenmiceandhumans,inbothspeciestherecognitionofCpGODNsismediatedbyTLR9.TheoptimalCpGmotifinhumansisGTCGTTandGACGTTforthemurinesequence.However,recentevidencesuggeststhatthissequencespecificityisrestrictedtophosphorothioate(PS)-modifiedODNandisnotobservedwhenanaturalphosphodiesterbackboneisused.Inrecentyearssequencerequirements,specificity,signalingpathwaysandkineticsoftheTLR9suppressionbyinhibitoryODNs(iODNs)havebeeninvestigated.