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Zyagen/IL11 Recombinant Protein/7938/1 Ea

Background

IL-11isamultifunctionalcytokineproducedbystromalcellssuchasfibroblasts,epithelialcellsandosteoclasts.Itisexpressedinawidevarietyoftissuesincludingthymus,lung,bone,connectivetissueandcentralnervoussystem.IL-11playsanimportantregulatoryroleinhematopoiesisbystimulatinggrowthofmyeloid,erythroidandmegakaryocyteprogenitorcells.Italsoregulatesbonemetabolism,inhibitsproductionofproinflammatorycytokinesandprotectsagainstgastromucosalinjury.RecombinantHumanIL-11isa19.1kDaproteinconsistingof179aminoacidresidues.

 

Specifications

AdditionalNames
AGIF(Adipogenesisinhibitoryfactor)
Preparation
TheED50 wasdeterminedbythedose-dependentstimulationoftheproliferationofmurineT11cellsis?2.0ng/ml,correspondingtoaspecificactivityof?5x105 units/mg.
Location
MPGPPPGPPRVSPDPRAELDSTVLLTRSLLADTRQLAAQLRDKFPADGDHNLDSLPTLAMSAGALGALQLPGVLTRLRADLLSYLRHVQWLRRAGGSSLKTLEPELGTLQARLDRLLRRLQLLMSRLALPQPPPDPPAPPLAPPSSAWGGIRAAHAILGGLHLTLDWAVRGLLLLKTRL
Source
E.coli
Reactivity
n/a
TestedApplication
n/a
Storage
ThelyophilizedIL-11recombinantproteinisstableforatleast2yearsfromdateofreceiptat-20ËšC.ReconstitutedIL-11isstableforatleast3monthswhenstoredinworkingaliquotswithacarrierproteinat-20ËšC.Aswithanyprotein,exposingIL-11recombinantproteintorepeatedfreeze/thawcyclesisnotrecommended.Whenworkingwithproteinscareshouldbetakentokeeprecombinantproteinatacoolandstabletemperature.
SpeciesReactivity
n/a
Purity
Greaterthan98%bySDS-PAGEgelandHPLCanalyses.

Endotoxinlevelislessthan0.1ngperμg(1EU/μg).

 


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