Adipogen/antiLAG3 (human), mAb (17B4) (ATTO 488)/AG20B0012TDT100/100 tests

MoreInformation
ProductDetails
Synonyms	LymphocyteActivationGene-3;FDCProtein;CD223
ProductType	MonoclonalAntibody
Properties
Clone	17B4
Isotype	MouseIgG1κ
Immunogen/Antigen	Syntheticpeptidecorrespondingto30aaintheN-terminusofhumanLAG-3.
Label/Conjugates	ATTO488
Application	FlowCytometry:(1:100) Immunocytochemistry Definitionfor1test:1μloflabeledantibodyin200μlofFlowCytometrybuffer(seeonlineprotocol).
Crossreactivity	Human
Specificity	RecognizeshumanLAG-3.
Purity	≥95%(SDS-PAGE)
PurityDetail	ProteinA-affinitypurified.
Concentration	1mg/ml
Formulation	Liquid.InPBScontaining0.02%sodiumazide.
OtherProductData	NewATTO-fluorescentantibodiesshowincreasedphotostability,outstandingbrightnessandintensesignals.ATTOdyesarethermallystable,resistanttoenvironmentalchangesandshownosignificantisomerization. ATTO488showsbrightgreenfluorescence(λabs(max):501nm,λem(max):523nm,εmax:90’000).
ShippingandHandling
Shipping	BLUEICE
ShortTermStorage	+4°C
LongTermStorage	+4°C
HandlingAdvice	Donotfreeze. Protectfromlight.
Use/Stability	Keepconjugatedformatsat+4°C. Stableforatleast1yearafterreceiptwhenstoredat+4°C.
Documents
Protocols	DownloadPDF
MSDS	DownloadPDF
ProductSpecificationSheet
Datasheet	DownloadPDF

Lymphocyteactivationgene3(LAG-3;CD223)playsanimportantroleinnegativelyregulatingTcellproliferation,functionandhomeostasis.ItisrequiredformaximalnaturalandinducedregulatoryTcell(Treg)function.LAG-3iscloselyrelatedtotheTcellco-receptorCD4andbindstoMHCclassIImoleculesbutwithasignificantlyhigheraffinitythanCD4.

ProductReferences

Characterizationofthelymphocyteactivationgene3-encodedprotein.AnewligandforhumanleukocyteantigenclassIIantigens:E.Baixeras,etal.;J.Exp.Med.176, 327(1992)
CellularexpressionandtissuedistributionofthehumanLAG-3-encodedprotein,anMHCclassIIligand:B.Huard,etal.;Immunogenetics 39, 213(1994)
CharacterizationofthemajorhistocompatibilitycomplexclassIIbindingsiteonLAG-3protein:B.Huard,etal.;PNAS 94, 5744(1997)
CD3/TCRcomplex-associatedlymphocyteactivationgene-3moleculesinhibitCD3/TCRsignaling:S.Hannier,etal.;J.Immunol. 161, 4058(1998)
TLymphocytesinfiltratingvarioustumourtypesexpresstheMHCclassIIligandlymphocyteactivationgene-3(LAG-3):roleofLAG-3/MHCclassIIinteractionsincell-cellcontacts:C.E.Demeure,etal.;Eur.J.Cancer 37, 1709(2001)
Immunologicalmechanismselicitedatthetumoursitebylymphocyteactivationgene-3(LAG-3)versusIL-12:sharingacommonTh1anti-tumourimmunepathway:E.D.Carlo,etal.;J.Pathol. 205, 82(2005)
Thenegativeregulatoryfunctionofthelymphocyte-activationgene-3co-receptor(CD223)onhumanTcells:L.Macon-LemaitreandF.Triebel;Immunology 115,170(2005)
Asolublelymphocyteactivationgene-3(sLAG-3)proteinasaprognosticfactorinhumanbreastcancerexpressingestrogenorprogesteronereceptors:F.Triebel,etal.;CancerLett. 235, 147(2006)
HumandendriticcellsacquireasemimaturephenotypeandlymphnodehomingpotentialthroughinteractionwithCD4+CD25+regulatoryTcells:J.Bayry,etal.;J.Immunol. 178, 4184(2007)
Humanlymphocyteactivationgene-3moleculesexpressedbyactivatedTcellsdelivercostimulationsignalfordendriticcellactivation:C.Casati,etal.;J.Immunol. 180, 3782(2008)

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Adipogen/antiLAG3 (human), mAb (17B4) (ATTO 488)/AG20B0012TDT100/100 tests