| Synonyms | Monoclonal C1S antibody, Anti-C1S antibody, Complement C1s antibody, C1S, CS 1, CS-1, CS 1 antibody, CS-1 antibody |
|---|---|
| Cross Reactivity | Human |
| Applications | WB |
| Immunogen | C1S antibody was raised in mouse using a full length recombinant protein of human C1S (NP_001725) produced in HEK293T cells, as the immunogen. |
| Host | Mouse |
|---|---|
| Clone | 4D10 |
| Isotype | IgG1 |
| Method of Purification | C1S antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
| Concentration | 500 ug-1 mg/ml |
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
|---|
| Usage Recommendations | WB: 1:2000 |
|---|
| Biological Significance | Activated C1s (a serine protease) cleaves two serum proteins C4 and C2. C4 is cleaved into a large fragment C4b, and a smaller, in active fragment C4a. C4b binds covalently to sugar residues on cell-surface glycoproteins, while C4a diffuses away following cleavage. C2 is cleaved into C2b and C2a. C2b binds noncovalently to a site on C4b, while C2a diffuses away. |
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