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This antibody recognizes a protein of 55-62kDa, identified as Cyclin B1.In mammals, Cyclin B1 associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at amino acids Thr-14 and Tyr-15.This maintains the inactive state until the end of G2-phase.The inactive Cyclin B1-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates amino acids Thr-14 and Tyr-15 of p34cdc2 rendering the complex active at the G2/M boundary.This mitotic kinase complex remains active until the metaphase/anaphase transition when Cyclin B1 is degraded.This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis.Cyclin B1-p34cdc2 plays a critical role in G2 to M transition.
The concentration stated for each application is a general starting point.Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
Hamster protein was used as the immunogen for this Cyclin B1 antibody.
Store the Cyclin B1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
CCNB, CCNB1, Cyclin B1 antibody
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