产品资料

Rockland/Anti-GAB1 Antibody/600-401-K05S/25 µL

Anti-GAB1AntibodyProperties

Description

Anti-GAB1(RABBIT)Antibody-600-401-K05S

Host

Rabbit

TargetSpecies

Human

KnownCrossReactivity

human

Clonality

Polyclonal

Application

ELISA:1:2,000-1:10,000
WesternBlot:1:500-1:2000
OtherDilution:UserOptimized

PhysicalState

Liquid(sterilefiltered)

ShippingCondition

DryIce

Format

IgG

Label

Unconjugated

Concentration

0.62mg/mLbyUVabsorbanceat280nm

Buffer

0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2

Preservative

0.01%(w/v)SodiumAzide

StABIlizer

None

GeneName

GAB1

RelevantLinks

GeneID-2549

Anti-GAB1AntibodyDescription

Background

GAB1antibodydetectshumanGAB1.GAB1isamemberoftheIRS1-likemultisubstratedockingproteinfamily.Theproteinisanimportantmediatorofbranchingtubulogenesisandplaysacentralroleincellulargrowthresponse,transformationandapoptosis.Twotranscriptvariantsencodingdifferentisoformshavebeenfoundforthisgene.GAB1playsaroleinintracellularsignalingcascadestriggeredbyactivatedreceptor-typekinases.ItisknowntoplayaroleinFGFR1signalingandisprobablyinvolvedinsignalingbytheepidermalgrowthfactorreceptor(EGFR)andtheinsulinreceptor(INSR).GAB1interactswithGRB2andwithotherSH2-containingproteins.ItisknowntointeractwithphosphorylatedLAT2,PTPRJ,FRS2,GRB2,PIK3R1andSOS1.GAB1getsphosphorylatedinresponsetoFGFR1activation.ThistyrosinephosphorylationofGAB1mediatesinteractionwithseveralproteinsthatcontainSH2domains.Anti-GAB1AntibodyisidealforinvestigatorsinvolvedinCellSignaling,Cancer,NeuroscienceandSignalTransductionresearch.

Synonyms

rabbitanti-GAB1antibody,GAB-1,GAB1,GRB2-associated-bindingprotein1,GRB2-associatedbinder1,Growthfactorreceptorboundprotein2-associatedprotein1

Immunogen

Anti-GAB1AntibodywasproducedinrabbitsbyrepeatedimmunizationswithasyntheticpeptidecorrespondingtoresiduessurroundingY659ofhumanGAB1protein.

ImmunogenType

Peptide

StorageCondition

Storevialat-20°Corbelowpriortoopening.Thisvialcontainsarelativelylowvolumeofreagent(25µL).Tominimizelossofvolumedilute1:10byadding225µLofthebufferstatedabovedirectlytothevial.Recap,mixthoroughlyandbrieflycentrifugetocollectthevolumeatthebottomofthevial.Usethisintermediatedilutionwhencalculatingfinaldilutionsasrecommendedbelow.Storethevialat-20°Corbelowafterdilution.Avoidcyclesoffreezingandthawing.

ApplicationNote

Anti-GAB1AntibodyhasbeentestedforuseinELISAandbywesternblot.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.Expectapredominantbandat76.6kDabywesternblottingintheappropriatecelllysateorextract.

Purity/Specifity

Anti-GAB1waspreparedfrommonospecificantiserumbyimmunoaffinitychromatographyusingphosphopeptidecoupledtoagarosebeadsfollowedbysolidphaseadsorptionsagainstnon-phosphopeptideandnon-specificpeptidetoremoveanyunwantedreactivities.Assaybyimmunoelectrophoresisresultedinasingleprecipitinarcagainstanti-RabbitSerum.ThisantibodyisspecificforhumanGAB1.Cross-reactivityagainstGAB1fromotherspeciesmayoccurbuthasnotyetbeentested.

DisclaimerNote-General

Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

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Rockland/Anti-GAB1 Antibody/600-401-K05S/25 µL

Anti-GAB1AntibodyProperties

Anti-GAB1AntibodyDescription