产品资料

Rockland/AKT2 Antibody/200-501-J35S/25 µL

AKT2AntibodyProperties

Description

Anti-AKT2(RAT)MonoclonalAntibody-200-501-J35S

Host

Rat

TargetSpecies

Human

KnownCrossReactivity

Human

Clonality

Monoclonal11F6.B2IgG2akappa

Application

ELISA:1:2,000-1:10,000
FlowCytometry:UserOptimized
WesternBlot:1:500-1:2000
Immunohistochemistry:20µg/mL
OtherDilution:UserOptimized

PhysicalState

Liquid(sterilefiltered)

ShippingCondition

DryIce

Format

IgG2a

Label

Unconjugated

Concentration

1.0mg/mlbyUVabsorbanceat280nm

Buffer

0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2

Preservative

0.01%(w/v)SodiumAzide

StABIlizer

None

GeneName

AKT2

RelevantLinks

UniProtKB-P31751
NCBI-P31751
GeneID-208

AKT2AntibodyDescription

Background

AKT2AntibodydetectsAKT2whichisacomponentofthePI-3kinasepathwayandisactivatedbyphosphorylationatSer473andThr308.AKTisacytoplasmicproteinalsoknownasProteinKinaseB(PKB)andrac(relatedtoAandCkinases).AKTisakeyregulatorofmanysignaltransductionpathways.AKTExhibitstightcontrolovercellproliferationandcellviability.OverexpressionorinappropriateactivationofAKTisnotedinmanytypesofcancer.AKTmediatesmanyofthedownstreameventsofPI3-kinase(alipidkinaseactivatedbygrowthfactors,cytokinesandinsulin).PI3-kinaserecruitsAKTtothemembrane,whereitisactivatedbyPDK1phosphorylation.Oncephosphorylated,AKTdissociatesfromthemembraneandphosphorylatestargetsinthecytoplasmandthecellnucleus.AKThastwomainroles:(i)inhibitionofapoptosis;(ii)promotionofproliferation.Anti-AKT2AntibodyisidealforinvestigatorsinvolvedinCellSignaling,Neuroscience,SignalTransductionresearch.

Synonyms

ratanti-Anti-AKT2antibody,AKT2antibody,AKT-2,PKBantibody,PKBbetaantibody,PKBBETAantibody,PRKBBantibody,ProteinkinaseAkt2antibody,ProteinkinaseBbetaantibody,RAC-betaserine/threonine-proteinkinase,RAC-PK-beta

Immunogen

Anti-AKT2AntibodywasproducedbyrepeatedimmunizationswithasyntheticpeptidecorrespondingtointernalresiduesofhumanAKT2protein.

ImmunogenType

Peptide

StorageCondition

Storevialat-20°Corbelowpriortoopening.Thisvialcontainsarelativelylowvolumeofreagent(25µL).Tominimizelossofvolumedilute1:10byadding225µLofthebufferstatedabovedirectlytothevial.Recap,mixthoroughlyandbrieflycentrifugetocollectthevolumeatthebottomofthevial.Usethisintermediatedilutionwhencalculatingfinaldilutionsasrecommendedbelow.Storethevialat-20°Corbelowafterdilution.Avoidcyclesoffreezingandthawing.

ApplicationNote

Anti-AKT2AntibodyissuitableforELISA,immunohistochemistry,andwesternblotting.Expectabandapproximately56kDainsizecorrespondingtoAKT2proteinbywesternblottingintheappropriatecelllysateorextract.ThismonoclonalantibodyreactswithhumanAKT.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.Forimmunohistochemistrywerecommendtheuseoffreshfrozentissues.Attemptsatstainingparaffin-embeddedformalinfixedtissueswerenegative.Nopre-treatmentofsampleisrequired.

Purity/Specifity

Anti-AKT2antibodyisdirectedagainsthumanAKT2.Theantibodydetectsbothunphosphorylatedandphosphorylatedformsoftheprotein.Anti-AKT2waspurifiedfromconcentratedtissueculturesupernatebyProteinAchromatography.CrossreactivitywithAKT2fromotherspecieshasnotbeendetermined,however,thesequenceoftheimmunogenshows88%identitytomouseand90%identitywithrat,therefore,crossreactivityisexpected.

DisclaimerNote-General

Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

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Rockland/AKT2 Antibody/200-501-J35S/25 µL

AKT2AntibodyProperties

AKT2AntibodyDescription