产品资料

Rockland/Alpha Amylase Antibody Peroxidase Conjugated/200-4345/20 mg

AlphaAmylaseAntibodyPeroxidaseConjugatedProperties

Description

Anti-ALPHAAMYLASE(Bacillusamyloliquefaciens)(RABBIT)AntibodyPeroxidaseConjugated-200-4345

Host

Rabbit

TargetSpecies

Bacillusamyloliquefaciens

KnownCrossReactivity

Bacillusamyloliquefaciens

Clonality

Polyclonal

Application

ELISA:1:5,000-1:20,000
WesternBlot:1:500-1:5,000
ImmunoPrecipitation:1:100
OtherDilution:UserOptimized

PhysicalState

Lyophilized

ShippingCondition

Ambient

Format

IgG

Label

Peroxidase(HorserADIsh)

Concentration

10.0mg/mLbyUVabsorbanceat280nm

Buffer

0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2

ReconstitutionVolume

2.0mL

ReconstitutionBuffer

Restorewithdeionizedwater(orequivalent)

Preservative

0.01%(w/v)GentamicinSulfate.DoNOTaddSodiumAzide!

StABIlizer

10mg/mLBovineSerumAlbumin(BSA)-ImmunoglobulinandProteasefree

GeneName

Alpha-amylase

RelevantLinks

UniProtKB
NCBI-P00692.1
UniProt-P00692
GeneID-7849308

AlphaAmylaseAntibodyPeroxidaseConjugatedDescription

Background

Anti-AlphaAmylaseAntibodydetectsAlphaAmylase.a-Amylaseisanenzymethathydrolysesalpha-bondsoflargealpha-linkedpolysaccharides,suchasstarchandglycogen,yieldingglucoseandmaltose.Anti-AlphaAmylaseAntibodyisidealforinvestigatorsinvolvedinCellSignaling.

Synonyms

Alpha-amylase,EC=3.2.1.1,1,4-alpha-D-glucanglucanohydrolase

Immunogen

Anti-AlphaAmylaseAntibodywasproducedbyrepeatedimmunizationswithBacillusamyloliquefaciensa-Amylaseprotein.

ImmunogenType

NativeProtein

StorageCondition

Storevialat4°Cpriortorestoration. Forextendedstoragealiquotcontentsandfreezeat-20°Corbelow. Avoidcyclesoffreezingandthawing. Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature. Thisproductisstableforseveralweeksat4°Casanundilutedliquid. Diluteonlypriortoimmediateuse.

ApplicationNote

AlphaAmylaseantibodyhasbeenassayedagainst1.0ugofAlphaAmylase[Bacillusamyloliquefaciens]inastandardcaptureELISAusingABTS(2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid])code#ABTS-100asasubstratefor30minutesatroomtemperature. Aworkingdilutionof1:2,000to1:10,000ofthereconstitutionconcentrationissuggestedforthisproduct.

Purity/Specifity

AlphaAmylaseAntibodyisanIgGfractionantibodypurifiedfrommonospecificantiserumbyamulti-stepprocesswhichincludesdelipidation,saltfractionationandionexchangechromatographyfollowedbyextensivedialysisagainstthebufferstatedabove. Assaybyimmunoelectrophoresisresultedinasingleprecipitinarcagainstanti-Peroxidase,anti-RabbitSerumaswellaspurifiedandpartiallypurifieda-Amylase[Bacillusamyloliquefaciens]. Crossreactivityagainsta-Amylasefromothertissuesandspeciesisunknown.

DisclaimerNote-General

Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX326,Gilbertsville,Pennsylvania,USA.

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Rockland/Alpha Amylase Antibody Peroxidase Conjugated/200-4345/20 mg

AlphaAmylaseAntibodyPeroxidaseConjugatedProperties

AlphaAmylaseAntibodyPeroxidaseConjugatedDescription