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产品资料

lumigen/Lumigen ECL Plus/PS3-100/

WesternBlottingHRPSubstrate

lumigenECLPlus(PS-3)isanacridan-basedtechnologychemiluminescentandchemifluorescentsubstrateforsensitivedetectionofWesternblottedproteinsthatareboundtohorserADIshperoxidase(HRP)labeledantibodies.

  • Highlysensitive - approximately5pgperbandoftargetproteinonWesternblot
  • Sustainedluminescence-signalissustainedforoverseveralhours
  • Dualsignalfunctionality-chemiluminescentandchemifluorescentsignalsaregeneratedsimultaneouslyallowingdetectiononx-rayfilmorwithadigitalimager

LumigenECLPlusTechnology

LumigenECLPlusutilizesauniqueacridanbasedtechnologyforthechemiluminescentandfluorescentdetectionofhorseradishperoxidase(HRP)conjugates.ThereactioninvolvestheenzymaticgenerationofthousandsofacridiniumesterintermediatesperminutewhichreactwithperoxideatalkalinepHtoproducesustainedhigh-intensitychemiluminescence.Additionally,thereactionend-productexhibitsalong-lastingfluorescentsignalwhichcanbedetectedonaCCDimagingdevice.

western blot

ecl extra

ProductSpecifications

Enzyme: 
HorseradishPeroxidase(HRP)
Application: 
WesternBlotting
Sensitivity: 
Lowpicogramtomidfemtogram
StorageConditions: 
2-8°C;Storeinamberbottletoprotectfromlight
StABIlity: 
2yearsstoredat2-8°C
WorkingSolution: 
40:1mixofAandBsolutions
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