产品资料

lumigen/Lumigen SPARCL/SDK-10K/

HomogeneousImmunoassay

lumigenSPARCLisahomogeneousproximityassaytechnologyutilizingflashchemiluminescencedetectionwithoutsolidsupportorwashstepsallowingforassaystobecompletedinlessthan30minutes.TheSPARCLtechnologyenablesassaystobeminiaturizedforhighthroughputscreeningwhilemaintainingsensitiveresultswithgooddynamicrange.SPARCLcanbeautomatedandadaptedformultipleapplicationsincludingELISA,protein-proteinandprotein-nucleicacidinteractions,andhighthroughputbindingassays.

Completeassaysinlessthan30minutes-Savetimewithonlyoneincubationstep,noseparationorwashstepsandinstantaneoussignalgenerationfromflashchemiluminescence
Costeffective-Usefewerreagentsandinstrumentswithnoneedforplate/beadcoatingoperations,automatedwashingstationsorotherexpensivedetectionequipment.
FlexIBLeassayformats-Adaptableformanydifferentkindsofassaysinsolutionorsolidphaseformatstostudyawidevarietyoftargets
Producelesswaste-Nowashmeansnodisposalofwashsolution.SPARCLproducesonly1xwellvolumeofwastecomparedto13xwellvolumeforconventionalELISAassays.

sparcl assay

SPARCLFormats

Solutionorsolidphaseassay
SPARCLcanbeimplementedinformatswithorwithoutasolidphase.Whenusingasolidphase,boththeacridancompoundandaspecificcaptureantibodyarecoupledtosolidphasessuchasmicroparticlesormicrotiterplates,whereaswhenthesolidphaseisomittedthecaptureantibodyisdirectlylabeledwiththeacridancompound.Thesolutionphaseformateliminatestheneedforplateorparticlecoating,improveskineticstoshortenincubationtimeandduetothelackofasolid/solutioninterfaceoffersamorenativeBIOLOGicalenvironment.

SPARCLTechnology

SPARCL(SpatialProximityAnalyteReagentCaptureLuminescence)isaproximitydependentchemiluminescenttechnologyforthedetectionofspecificbindinginteractionorassociationbetweentwobindingpartners.InaSPARCLassay,abindingpartnerlabeledwithachemiluminescentsubstrate(acridan)andasecondbindingpartnerlabeledwithhorserADIshperoxidase(HRP)arebroughtintocloseproximitytoeachotherthroughaspecificbindingevent.BecauseofthiscloseproximityofacridantotheHRPenzymeaflashofchemiluminescenceisgenerateduponadditionofatriggersolutioncontaininghydrogenperoxideandanenhancer.

homogeneous immunoassay

SPARCL™–AsimplerandfasterELISAalternative

Fast,SimpleandCost-effectiveImmunoassayDevelopment

ProductSpecifications

Enzyme:

HorseradishPeroxidase(HRP)

Application:

ELISA,ProximityAssays

Sensitivity:

Lowpicogram

StorageConditions:

2-8°C;Storeinamberbottletoprotectfromlight

StABIlity:

1yearstoredat2-8°C

WorkingSolution:

Readytousesolution