产品资料

ProteinTech/IFT88 Polyclonal antibody/150ul/13967-1-AP

Tested Applications

Positive WB detected in	HEK-293 cells, Jurkat cells,MDCK cells,NIH/3T3 cells
Positive IP detected in	knockout cells and WT cells,HEK-293 cells
Positive IHC detected in	human heart tissue, human pancreas tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in	MDCK cells, hTERT-RPE1 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)	IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 27 publications below
WB	See 84 publications below
IHC	See 1 publications below
IF	See 159 publications below
IP	See 2 publications below
CoIP	See 1 publications below

Product Information

13967-1-AP targets IFT88 inWB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat,dog samples.

Tested Reactivity	human, mouse, rat,dog
Cited Reactivity	Chick, Chicken, dog, human, mouse, rat, swine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	IFT88 fusion protein Ag4980
Full Name	intraflagellar transport 88 homolog (Chlamydomonas)
Calculated molecular weight	94 kDa
Observed molecular weight	88-95 kDa
GenBank accession number	BC030776
Gene symbol	IFT88
Gene ID (NCBI)	8100
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20^oC storage.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926). This antibody was raised against the C-terminal region of human IFT88 and can detect the endogenous level of IFT88.

Protocols

Product Specific Protocols
WB protocol for IFT88 antibody 13967-1-AP	Download protocol
IHC protocol for IFT88 antibody 13967-1-AP	Download protocol
IF protocol for IFT88 antibody 13967-1-AP	Download protocol
IP protocol for IFT88 antibody 13967-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols