产品资料

ProteinTech/GAPDH Monoclonal antibody/150ul/60004-1-Ig

Tested Applications

Positive WB detected in	HeLa cells, soybean whole plant tissue,arabidopsis whole plant tissue,HepG2 cells,ROS1728 cells,pig brain tissue,zebrafish tissue,whole yeast cells,whole Nematode tissue,HEK-293 cells,HSC-T6 cells,PC-12 cells,NIH/3T3 cells,C2C12 cells,SP2/0 cells,rat brain tissue,mouse brain tissue
Positive IP detected in	HeLa Cells
Positive IF detected in	Ethacrynic acid treated HeLa cells
Positive FC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:20000-1:100000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for IP and 1:2000-1:12000 for WB
Immunofluorescence (IF)	IF : 1:200-1:2000
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 1 publications below
WB	See 2758 publications below
IHC	See 2 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

60004-1-Ig targets GAPDH inWB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human,mouse,rat,yeast, plant samples.

Tested Reactivity	human,mouse,rat,yeast, plant
Cited Reactivity	A. flavus, azalea R. hainanense, beagle, Bovine, canine, carp, chicken, cow, Cynomorium songaricum Rupr, cyprinid
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	GAPDH fusion protein Ag0766
Full Name	glyceraldehyde-3-phosphate dehydrogenase
Calculated molecular weight	36kDa
Observed molecular weight	36 kDa
GenBank accession number	BC004109
Gene symbol	GAPDH
Gene ID (NCBI)	2597
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20^oC storage.

Background Information

WhatisthemolecularweightofGAPDH?

ThemolecularweightofGAPDHis36kD.

WhatisGAPDH?

GAPDHstandsforglyceraldehyde-3-phosphatedehydrogenaseandisoftenreferredtoasa“housekeeping”

protein.Itisrecognizedasanimportantenzymeinvolvedinmetabolicpathways,aidingintheglycolytic

productionofATPandpyruvate(PMID:21895736).

WhatisthefunctionofGAPDH?

GAPDHisresponsibleforcatalyzingthephosphorylationofglyceraldehyde-3-phosphateintoD-glycerate-1,3-biphosphate.WhilethisfunctionofGAPDHisconsidereditsprimaryfunction,GAPDHisalsoinvolved,even

critical,inothercellfunctions,someofwhicharestillbeingstudied.RecentstudieshavesuggestedthatGAPDH

isakeyfactorinregulatingapoptosis,translocatingtothenucleusundercertainstressfulconditionsandacting

asasignalingfactorinoxidativestress-inducedapoptosis.OtherstudiesindicatethatGAPDHplaysarolein

transportingvesiclesfromtheendoplasmicreticulumtotheGolgibody(PMIDs:21895736,12829261,25859407,

11035021).

WhereinthecellisGAPDHlocalized?

GAPDHisexpressedprimarilyinthecytosolicandmembraneregions,withminimalexpressioninthenucleus

(PMIDs:21895736,12829261).

IsthereareasontouseanantibodytargetingGAPDHifmyresearchisnotconcernedwithglycolysisornuclear

functions?

Yes!BecauseGAPDHisoftenhighlyexpressedthroughoutdifferenttissuesandcelltypes,GAPDHexpressionis

usefulasacontrolforWesternblotsandRT-PCR.

Whataretheknownprotein-proteininteractionsofGAPDH?

GAPDHinteractswithotherproteinstoregulatenucleartranslocationandGAPDHcatalyticactivity.Someof

theseproteinsincludeTPPP,SIAH1,CHP1,RILPL1/GOSPELandFKBP6(PMIDs:21895736,25859407).

Note:For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable.

Protocols

Product Specific Protocols
WB protocol for GAPDH antibody 60004-1-Ig	Download protocol
IF protocol for GAPDH antibody 60004-1-Ig	Download protocol
FC protocol for GAPDH antibody 60004-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols