产品资料

ProteinTech/Beta Actin Monoclonal antibody/150ul/60008-1-Ig

Tested Applications

Positive WB detected in	multi-cells/tissue, HeLa cells,MCF-7 cells,HEK-293 cells,A549 cells,rice whole plant tissue,arabidopsis whole plant tissue
Positive IHC detected in	human colon tissue, human brain tissue,human kidney tissue,human lung tissue,human spleen tissue,human ovary tissue,human testis tissue,human placenta tissue,human heart tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in	HepG2 cells
Positive FC detected in	HepG2 cells

Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WB	See 1739 publications below
IHC	See 1 publications below
IF	See 2 publications below

Product Information

60008-1-Ig targets Beta Actin inWB, IHC, IF, FC, ELISA applications and shows reactivity with human,mouse,rat,pig, plant, Zebrafish samples.

Tested Reactivity	human,mouse,rat,pig, plant, Zebrafish
Cited Reactivity	A. sinica, canine, cat, Chicken, cow, Drosophila, Drosophila melanogaster, duck, eels, Eelworm
Host / Isotype	Mouse / IgM
Class	Monoclonal
Type	Antibody
Immunogen	Beta Actin fusion protein Ag0297
Full Name	actin, beta
Calculated molecular weight	375 aa, 42 kDa
Observed molecular weight	42 kDa
GenBank accession number	BC002409
Gene symbol	ACTB
Gene ID (NCBI)	60
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20^oC storage.

Background Information

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.

Protocols

Product Specific Protocols
WB protocol for Beta Actin antibody 60008-1-Ig	Download protocol
IHC protocol for Beta Actin antibody 60008-1-Ig	Download protocol
IF protocol for Beta Actin antibody 60008-1-Ig	Download protocol
FC protocol for Beta Actin antibody 60008-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols