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| Positive WB detected in | rat skeletal muscle tissue, mouse skeletal muscle tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human colon tissue,human heart tissue,human skeletal muscle tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:8000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
| WB | See 17 publications below |
| IF | See 4 publications below |
| FC | See 1 publications below |
The immunogen of 23660-1-AP is Alpha Actin Fusion Protein expressed in E. coli.
| Tested Reactivity | human,mouse,rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Alpha Actin fusion protein Ag19151 |
| Full Name | actin, alpha 1, skeletal muscle |
| Calculated molecular weight | 42 kDa |
| Observed molecular weight | 40-42 kDa |
| GenBank accession number | BC012597 |
| Gene symbol | ACTA1 |
| Gene ID (NCBI) | 58 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage. |
| Product Specific Protocols | |
|---|---|
| WB protocol for Alpha Actin antibody 23660-1-AP | Download protocol |
| IHC protocol for Alpha Actin antibody 23660-1-AP | Download protocol |
| IF protocol for Alpha Actin antibody 23660-1-AP | Download protocol |
| IP protocol for Alpha Actin antibody 23660-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
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