产品资料

ProteinTech/Lamin B1 Polyclonal antibody/150ul/12987-1-AP

Tested Applications

Positive WB detected in	HeLa cells, U-937 cells,mouse spleen tissue,mouse pancreas tissue,mouse lung tissue,SH-SY5Y cells,K-562 cells,Y79 cells,mouse eye tissue,mouse kidney tissue,NIH/3T3 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human liver cancer tissue, human colon cancer tissue,human kidney tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in	HepG2 cells, human skin cancer tissue,NIH/3T3 cells
Positive FC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WB	See 333 publications below
IHC	See 1 publications below
IF	See 11 publications below
ChIP	See 1 publications below

Product Information

12987-1-AP targets Lamin B1 inWB, IP, IHC, IF, FC, CHIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	canine, chicken, hamster, human, mouse, rat, swine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Lamin B1 fusion protein Ag3631
Full Name	lamin B1
Calculated molecular weight	66 kDa
Observed molecular weight	66-70 kDa
GenBank accession number	BC012295
Gene symbol	LMNB1
Gene ID (NCBI)	4001
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20^oC storage.

Background Information

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Expression of uncleavable mutant lamin A or B caused significant delays in the onset of chromatin condensation and nuclear shrinkage during apoptosis (PMID:11953316). This protein is not suitable for samples where the nuclear envelope has been removed.

Protocols

Product Specific Protocols
WB protocol for Lamin B1 antibody 12987-1-AP	Download protocol
IHC protocol for Lamin B1 antibody 12987-1-AP	Download protocol
IF protocol for Lamin B1 antibody 12987-1-AP	Download protocol
IP protocol for Lamin B1 antibody 12987-1-AP	Download protocol
FC protocol for Lamin B1 antibody 12987-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols