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| Positive WB detected in | SGC-7901 cells |
| Positive IHC detected in | human pancreas cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
11856-1-AP targets Caspase 4/p20/p10 inWB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Caspase 4/p20/p10 fusion protein Ag2429 |
| Full Name | caspase 4, apoptosis-related cysteine peptidase |
| Calculated molecular weight | 377 aa, 43 kDa |
| Observed molecular weight | 43-48 kDa |
| GenBank accession number | BC017839 |
| Gene symbol | CASP4 |
| Gene ID (NCBI) | 837 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage. |
Caspase 4 (CASP4),is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.Caspase 4 is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. Overexpression of caspase 4 will induce cell apoptosis.
| Product Specific Protocols | |
|---|---|
| WB protocol for Caspase 4/p20/p10 antibody 11856-1-AP | Download protocol |
| IHC protocol for Caspase 4/p20/p10 antibody 11856-1-AP | Download protocol |
| IF protocol for Caspase 4/p20/p10 antibody 11856-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
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