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| Positive WB detected in | A431 cells, HeLa cells,human brain tissue,Tunicamycin treated HeLa cells,A549 cells,HEK-293 cells,HepG2 cells,Jurkat cells |
| Positive IP detected in | HeLa cells |
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Sample-dependent, check data in validation data gallery | |
| WB | See 32 publications below |
10988-1-AP targets BID inWB, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | BID fusion protein Ag1445 |
| Full Name | BH3 interacting domain death agonist |
| Calculated molecular weight | 22 kDa |
| Observed molecular weight | 22 kDa |
| GenBank accession number | BC009197 |
| Gene symbol | BID |
| Gene ID (NCBI) | 637 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage. |
BID, also named as p22 BID, can be cleaved into p11 BID, p13 BID and p15 BID. It is pro-apoptotic molecules. The major proteolytic product p15 BID allows the release of cytochrome c. BID-L, BID-EL and BID-ES induce ICE-like proteases and apoptosis. BID-S does not induce apoptosis. BID counters the protective effect of Bcl-2. (PMID:14583606)
| Product Specific Protocols | |
|---|---|
| WB protocol for BID antibody 10988-1-AP | Download protocol |
| IP protocol for BID antibody 10988-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
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