Serum-SalivaCorrelation | 0.75-0.96 |
Sensitivity | 35pg/mL |
SampleTestVolume | 100µl |
RecommendedCollectionVolume | 175µl |
SpecialConsiderations | Postureaffectslevels-werecommendsamplingafter30minutesinposition. Licorice(real)mimicsaldosteroneandshouldbeavoided2weeksbeforecollection. |
CollectionProtocol | DownloadPDF |
Recentstudieshaveshownthatprimaryaldosteronism(PA)isthemostcommoncauseofsecondaryhypertensionwithaprevalenceofapproximately5-10%amongallhypertensivepatientsandanevenhigherprevalenceamongselectedpatientswithadvancedstagesofhypertensionandresistanthypertension.(1) ScreeningforPAamonghypertensivepatientsisimportantdue toitsassociationwithriskforcardiovasculardiseaseandrenaldamage,(1)andnon-invasivesalivaryaldosteronemeasurementshaverecentlybeenexploredasameanstofacilitatethisscreening. MorningsalivaryaldosteronemeasurementsalonewerefoundtohavesomeABIlitytodiscriminatebetweenpatientswithPAandessentialhypertension,andthepresenceorabsenceofadiurnaldeclineshowedpromisefordistinguishingbetweenthetwoformsofthedisease(adenomasvs.bilateralhyperplasia).(2)
Dysregulationofcirculatingaldosteronelevelshasalsobeenassociatedwithpsychiatricdisorders,andarecentstudyhassimilarlyreportedasignificantnegativeassociationbetweenmorningsalivaryaldosteronelevelsandtraitanxietyscores. Thosewiththehighanxietytraitmaybeassociated withaninabilitytorespondwithadequatecortisollevelsduringstress.(3)
Producedlargelyintheadrenal glands,aldosteroneisclassifiedasamineralocorticoidsteroidsinceitsclassicaleffectistoregulatethetransportofsodiumandwateracrosscellsofthekidneyinexchangeforpotassiumandhydrogenions,therebyregulatingbloodvolumeandpressure.(4,5)
Additionally,rapidnon-genomicactionsandlocalproductionofaldosteronehavebeenidentifiedinothertissues,includingtheheart,vascularsystem,adrenalgland,andkidney. Thesenon-genomicmechanismsarebeingstudiedinconnectionwitharangeofdiseasesincludingcardiovasculardisease,cirrhosis,kidneydisease,insulinresistance,anddiabetes.(6,7,8)
Nospecificbindingproteinforaldosteronehasbeenidentifiedinblood.(4) Circulatingaldosteronenotboundtoserumproteinsenterssalivabypassivediffusion.(9) Salivaryaldosteronelevelscorrespondapproximatelyto30%ofthosefoundinplasma,withgoodcorrelationfoundbetweenplasmaandnon-extractedsalivaryaldosterone.(10) Salivaryaldosteronelevelsareunaffectedbysalivaryflowrateorhormone-bindingproteins.(11) Bothsalivaryandplasmaaldosteroneincreasesignificantlywhilestanding,comparedtobeingseated;thiseffectissignificantlyhigherinfemalesthaninmales. Adiurnalrhythmforsalivaryaldosteroneexistsforhealthyindividuals,withhighestlevelsinthemorning.(10)
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn