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TheCultreCoat®VascularPermeABIlityAssayemploysasimplifiedBoydenchamberdesignwithaonemicronpolyethyleneterephthalate(PET)membranecoatedwithcollagenI.Portswithinthemigrationchamber(top)allowaccesstotheassaychamber(bottom)withoutdismantlingthedevice.Thisdesigniseasiertouse,preventscontamination,andisadaptableforrobotichighthroughputsystems.Theassaychambermaybedirectlyanalyzedinaplatereader,eliminatingtransferstepsthatintroduceadditionalvariabilitytotheassay.
Theconfluentcellmonolayerformsabarrierthroughcell-celladhesionmechanismssuchasjunctions(adherens,tight,andgap)anddesmosomes,andthisbarrierrestrictsFITC-Dextrantotheinserts.Disruptionofthesecell-celladhesionmechanismscreatesgapsintheintercellularspacethatallowdiffusionofFITC-Dextranintothebottomchamber,sotheamountofFITC-Dextranthatdiffusesisrelatedtotheamountofintercellularspace,whichisrepresentativeofvascularpermeability.
| Catalog#3475-096-Kincludes: | ||
| CatalogNumber | Description | Qty |
| 3475-096-01 | CultreCoat®96WellVascularPermeabilityChamber | 1 |
| 3475-096-02 | CultreCoat®FITC-Dextran | 1 |
| Catalog#3475-024-Kincludes: | ||
| CatalogNumber | Description | Qty |
| 3475-024-01 | CultreCoat®24WellVascularPermeabilityChamber | 1 |
| 3475-096-02 | CultreCoat®FITC-Dextran | 1 |
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn