TheCultreCoat®VascularPermeABIlityAssayemploysasimplifiedBoydenchamberdesignwithaonemicronpolyethyleneterephthalate(PET)membranecoatedwithcollagenI.Portswithinthemigrationchamber(top)allowaccesstotheassaychamber(bottom)withoutdismantlingthedevice.Thisdesigniseasiertouse,preventscontamination,andisadaptableforrobotichighthroughputsystems.Theassaychambermaybedirectlyanalyzedinaplatereader,eliminatingtransferstepsthatintroduceadditionalvariabilitytotheassay.
Theconfluentcellmonolayerformsabarrierthroughcell-celladhesionmechanismssuchasjunctions(adherens,tight,andgap)anddesmosomes,andthisbarrierrestrictsFITC-Dextrantotheinserts.Disruptionofthesecell-celladhesionmechanismscreatesgapsintheintercellularspacethatallowdiffusionofFITC-Dextranintothebottomchamber,sotheamountofFITC-Dextranthatdiffusesisrelatedtotheamountofintercellularspace,whichisrepresentativeofvascularpermeability.
| Catalog#3475-096-Kincludes: | ||
| CatalogNumber | Description | Qty |
| 3475-096-01 | CultreCoat®96WellVascularPermeabilityChamber | 1 |
| 3475-096-02 | CultreCoat®FITC-Dextran | 1 |
| Catalog#3475-024-Kincludes: | ||
| CatalogNumber | Description | Qty |
| 3475-024-01 | CultreCoat®24WellVascularPermeabilityChamber | 1 |
| 3475-096-02 | CultreCoat®FITC-Dextran | 1 |
matriks/Shikari® (S-ATP) Anti-Pembrolizumab ELISA w/confirmation/PEM-QNS-KEY
intactgenomics/Methionine Auxotrophic LBA4404 | ig® | Intact Genomics/15x50μl/1076-15
goprolytix/Proteolytic Activation of Prothrombin/1 mg, 200 µg/BCT-DFP
novateinbio/Dynorphin A ( 13-17 ) Porcine Peptide/5mg/PE-54024_5mg
matriks/Shikari® (Q-ETA) Etanercept ELISA/ETA-FD-ENB
intactgenomics/Agrobacterium ElectroComponent Combo Pack | Intact Genomics/12x50µl/1290-24
Jackson/Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)/2.0 ml/115-035-003
Jackson/Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)/2.0 ml/111-035-003
Qubit™ dsDNA BR Assay Kit Q32853现货
Invitrogen Q32855 Qubit™ RNA HS Assay Kit现货促销
LUCK-1G,D-Luciferin, Potassium Salt 钾盐现货
GoldBio/D-Luciferin, Sodium Salt (Proven and Published)/LUCNA-1G/1 g